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Function of chlorophyll in photosynthesis
It absorbs light energy and converts it into chemical energy.
Two main reasons most life depends on photosynthesis
It provides an energy source and a source of oxygen.
Word equation for photosynthesis
Carbon dioxide + water → glucose + oxygen (light energy, chlorophyll)
Type of energy light is converted into during photosynthesis
Chemical energy
Two ways to determine the rate of photosynthesis
Measure the decrease in a reactant or the increase in a product.
Glucose use NOT done by plants (MCQ)
C) Production of oxygen
Factor NOT affecting photosynthesis rate (MCQ)
D) Humidity
How to measure amount of oxygen produced in photosynthesis (2 ways)
Count bubbles over time or measure oxygen volume using a gas syringe.
Energy conversion during photosynthesis
Light energy → Chemical energy
Type of energy chlorophyll traps
Light energy
Uses of glucose produced from photosynthesis (CASFM)
Converted to cellulose (cell walls), amino acids (proteins), sucrose or starch (storage), fats, or oxidized for energy in respiration.
Importance of photosynthesis
Source of food/energy; removes CO₂ and produces O₂.
Tool to measure CO₂ concentration decrease
Hydrogen carbonate indicator
How hydrogen carbonate indicator works
Color changes based on CO₂ concentration; observe over fixed time.
How to test for starch in leaves
Use iodine solution on a decolorised leaf to observe color change.
Steps for starch test (iodine test)
Destarch plant → Boil leaf in water → Boil in ethanol in hot water → Wash leaf → Place on tile → Add iodine → Observe color change
Positive result for iodine test (starch present)
Solution turns blue-black
Negative result for iodine test (no starch)
Solution stays yellowish-brown
Factors affecting rate of photosynthesis
Light intensity, CO₂ concentration, and temperature
How to vary light intensity in experiments
Change distance of lamp from plant setup
How to vary temperature in experiments
Use water bath; adjust with hot or cold water
How to vary CO₂ concentration in experiments
Adjust sodium hydrogen carbonate concentration
Light intensity and ROP relationship
Higher light intensity → Higher ROP due to more light energy
CO₂ concentration and ROP relationship
Higher CO₂ → Higher ROP since CO₂ is a photosynthesis substrate
Temperature and ROP relationship (Stage 1 - Low temp)
Low temp → Low ROP due to inactive enzymes and fewer collisions
Temperature and ROP relationship (Stage 2 - Increasing temp)
Higher temp → Higher ROP due to more kinetic energy and collisions
Temperature and ROP relationship (Stage 3 - Optimum exceeded)
ROP peaks at optimum temp; above that, enzymes denature and ROP decreases