MLT 101: Microscope Lesson

These flashcards cover key terms and concepts from the lecture on hematology, including blood cell types, functions, and relevant processes.

The difference between a simple microscope and a compound microscope

The simple microscope is a magnifying glass with the sun being the light source. The Compound Microscope has two separate lenses with the light source being the light bulb

The calculation for Total magnification of a compound microscope

Total Magnification= Magnification of Objective lens X Magnification of the Eye Piece

Refractive Index

the amount of speed in which light bounces from the lens to the specimen. This is enhanced through the immersion oil.

Resolving Power

The ability of a microscope at a specific magnification to distinguish between 2 specific objects that are situated closely together. For example, the difference between an Apple Camera and an old Polaroid Camera

Depth of Field

The ability of the objective lens to focus through different planes

Magnification

The process of enlarging the apparent size, not physical size. Expressed as 10x the actual size or 100x the actual size and so forth

Par Focal Lens

a lens that stays in focus when magnification/focal length is changed. So as the objective switches, the lens stays in focus

Stage

A place where the slide sits

Base

A place where the light adjuster and the power switch is underneath. This is the bottom of the microscope

Diaphragm

A place where the light increases or decreases

Condenser

Focuses light onto the specimen for clarity and contrast.

Eyepiece (or Ocular Lens)

A place where you put your eyes onto to view the specimen

Objective Lens

The lens that helps you focus on the specimen with magnification and resolution. Has X10, X40 and X100-immersion oil objectives.

Light source of the microscope

The Light Bulb, found somewhere at the base of the microscope near the Illuminator

Coarse Adjustment Knob

The big knob that gives a rough focus on the specimen

Fine Adjustment Knob

The smaller knob outside the Coarse adjustment knob that fine tunes the view of the specimen

Condenser Adjustment Knob

This is the knob on the side beneath the stage that adjusts the beam of light up or down

How to properly clean the microscope

Please use lens paper to wipe the microscope and its lens. Use the lens cleaner and the lens paper to get the immersion oil off

Why we do not leave immersion oil on any lens of the microscope

Immersion oil dries and seeps under the objective lens