MICROSCOPE HISTO 1

Rules for Microscope Use

Carry with two hands, clean lenses with lens paper only, do not force adjustment knobs, always cover after use, handle cords carefully.

Light Microscope

Uses light to view samples.

Electron Microscope

Uses electron beams for higher magnification.

Eyepiece

Magnifies image from the objective lens, usually 10x.

Nosepiece

Holds and rotates objective lenses.

Objectives

Lenses with varying magnifications: Scanning (4x, red), Low Power (10x, yellow), High Power (40x, blue).

Stage

Platform where the slide is placed.

Coarse Focus Knob

Used for large adjustments when focusing.

Fine Focus Knob

Used for detailed focusing.

Total Magnification

Calculated by multiplying eyepiece magnification by objective lens magnification.

Steps to Use the Microscope

1. Place slide on stage and secure with clips. 2. Start with lowest power objective. 3. Look through eyepiece. 4. Use Coarse Focus, then Fine Focus. 5. Switch to higher powers as needed.

Fixation

Preserves tissues using chemicals for microscopy.

Staining

Involves using dyes like Hematoxylin to stain basophilic structures and Eosin to stain acidophilic structures.

Transmission Electron Microscope (TEM)

Produces a 2D image by passing an electron beam through the specimen.

Scanning Electron Microscope (SEM)

Produces a 3D image by bouncing an electron beam off the specimen surface.