PRECIPITATION AND AGGLUTINATION REACTION

IMMUNOASSAYS

This have been developed to detect either antigen or antibody, and they vary fromeasily performed manual tests to highly complex automated assays.

PRECIPITATION

It involves combining soluble antigen with soluble antibody to produce insolublecomplexes that are visible.

It deals with a small soluble antigen that reacts with a small soluble antibody.

Contains small molecules

SMALL ANTIGEN

small, free-floating antigen that reacts with smallantibodies as well.

IgG

Most efficient in this reaction. (Precipitation)

IgM

Can also perform precipitation

Liquid Medium

We are able to observe the reaction in a ______ _______.

AGGLUTINATION

_________ is the process by which particulate antigens aggregate to form larger complexes when a specific antibody is present.

PARTICULATE ANTIGENS

____________ ___________ antigens mainly found on the surface of a particle. These particles can be naturally or synthetically attached to a carrier particle.

PARTICULATE ANTIGENS

____________ ___________ antigens mainly found on the surface of a particle. These particles can be naturally or synthetically attached to a carrier particle.

IgM

Most efficient in this reaction. (Agglutination)

AGGLUTINATION AND HEMOLYSIS

In terms of blood banking, most of its routine tests are either (a).____________ or (b)___________.

(a) ___________ and (b)____________ is always a positive reaction.

Note: the first (a) is the same as the 2nd (a).

IgG

_____ can also perform this but it needs to be incubated at 37 deg C to enhance the agglutination reaction.