histopath lab - infiltration & embedding

To replace the clearing agent with a medium that provides support for sectioning

What is the main purpose of infiltration in tissue processing?

Paraffin wax

Which of the following is the most commonly used infiltration medium in histopathology?

45–60°C

What is the ideal temperature range for paraffin infiltration?

Tissue hardens and becomes brittle

What happens if paraffin temperature is too high during infiltration?

Xylene

Which clearing agent is commonly used before paraffin infiltration?

Decrease paraffin infiltration time and improve penetration

The main purpose of vacuum embedding is to:

Tissue will not be properly supported, leading to poor sectioning

What will happen if infiltration time is too short?

To harden tissues for sectioning

What is the primary purpose of embedding?

Xylene

Which of the following is not a commonly used embedding medium?

Paraffin embedding

What is the most common embedding technique used in routine histopathology?

At the bottom of the mold

When embedding a tissue block, the cutting surface of the tissue should be placed:

Epoxy resin

Which embedding material is used for electron microscopy?

Splitting and crumbling of sections

Which artifact is caused by incomplete infiltration?

All of the above

Which of the following can cause tissue brittleness?
a) Prolonged clearing with xylene
b) Excessive dehydration
c) High infiltration temperature
d) All of the above

Improper handling during embedding

If tissue blocks contain air bubbles after embedding, what is the most likely cause?

Plastic embedding

Which type of embedding is used for hard tissues like bone?

They do not require dehydration or clearing

What is the main advantage of using water-soluble waxes in embedding?

Celloidin and paraffin

In double embedding, which two media are used?

To remove excess wax and expose the tissue surface for sectioning

What is the purpose of trimming after embedding?

It removes wrinkles from the sections

What is the importance of floating out tissue sections after trimming?