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DNA structure
deoxyribose nucleic acid in an alpha helix
made up of sugars and phosphates
antiparallel
the two strands of DNA run in opposite directions
what is RNA
single strand of DNA, consists of single bases
made of sugars (ribose) and phosphates
no thymine, instead there is uracil
messenger RNA/mRNA
coded by an enzyme as a complimentary copy of the gene
ribosomal RNA/rRNA
coils to make a ribosome
binds to mRNA when trying to make a polypeptise chain
transfer RNA/tRNA
carries a different amino acid to the codon to code for things
gene
a section of a chromosome that codes for one characteristic
DNA replication
in the S phase of cell life, the amount of DNA doubles
semiconservative
2 new strands are made to pair with the 2 old strands
helicase
an enzyme that separates the two strands of DNA
DNA polymerase
adds bases to each of the old strand and makes two new paired strands
endonuclease
proofreads the new DNA stand
exonuclease
cuts out anything in the new DNA strand put their by mistake
ligase
connects broek pieces of the DNA strand together
protein synthesis stages
transcription and translation
transcription
inside of the nucleus, DNA unwinds and separates
RNA polymerase puts a copy of the gene onto the mRNA
mRNA leaves the nucleus
translation
mRNA binds to a ribosome
tRNA carries different amino acids to the ribosome to mae the protein coded by mRNA
ribosome rease the codons in threes
genetic code
based on the 3-base sequence of the mRNA
eukaryotic gene expression
a cap is placed on one end of the mRNA and a tail is put on the other end
DNA contains introns and exons
prokaryotic gene expression
do not have introns, only contain exons
introns
sections within a gene that code for nothing and are cut out
exons
genes to code the message
quorum sensing
when bacteria releases a molecules that helps ot determine how big their population is together
helps them survive and form biofilms
antigenic variation
altering surface proteins help them evade the immune system
operon
a group of genes that work together and a control section that turns them on and off
two kinds: lac operon and trp operon
lac operon
consists of three genes for making an enzyme to break down lactose and a control sections to activate and deactivate themselves
trp operon
5 genes and a control section
code for making the amino acid tryptophan
the genes are always on and making tryptophan, but if there are too many the control section shuts down