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What is serum
plasma with clotting factors removed
Clotting factors
fibrinogen
Serum protein concentration
60-80 mg/ml
Albumins, globulins
Sera used in cell cultures
Bovine fetus
New-born calf
Human
Horse
Pig
Goat
Chicken
NuSerum
Synthetic serum replacement; more chemically defined; low protein
Functions of Serum
Promote attachment and spreading of anchorage dependent
Provides source of minerals, lipids, aa, fatty acids, carbs
Contains growth factors, hormones
Contains trypsin inhibitors
Drawbacks of serum
Variability
difference in quantity and quality of components
Shelf Life
no freeze-thaw
One year at -80
Quality and Standardization
varies with lots or batches
Specificity
Availability and cost
Contamination