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Plasma Membrane
The cell boundary that separates internal conditions from the external environment.
Nucleus and Nuclear Envelope
The DNA-containing control center enclosed by a double membrane with pores continuous with the ER.
Ribosomes
Nonmembranous rRNA-protein complexes that translate mRNA sequences into polypeptides.
Free vs. Bound Ribosomes
Free make cytosolic proteins; bound on rough ER make secreted or membrane proteins.
Endomembrane System
A network of membranes that modifies, packages, and transports proteins, lipids, and polysaccharides.
Transport Vesicles
Small membrane sacs that move materials between organelles in the endomembrane system.
Golgi Apparatus
A stack of flattened sacs that modifies, sorts, folds, and packages cellular products.
Mitochondria
Double-membraned organelles that carry out aerobic respiration and produce most cellular ATP.
Lysosomes
Membrane-bound sacs of hydrolytic enzymes that digest materials and recycle cell components.
Chloroplasts
Double-membraned organelles in plants and algae where photosynthesis converts light energy to chemical energy.
Prokaryotic vs. Eukaryotic Cells
Prokaryotes lack membrane-bound organelles and a nucleus; eukaryotes contain both.
Plant vs. Animal Cells
Plant cells have cell walls and chloroplasts; animal cells lack them and typically have centrioles.
Endoplasmic Reticulum (Rough and Smooth)
A membrane network that supports cell shape, transports materials, and synthesizes proteins or lipids.
Vacuoles
Membrane-bound sacs that store materials; plant central vacuoles maintain turgor pressure.
Surface Area-to-Volume Ratio
The amount of membrane surface available for exchange relative to internal cell volume.
Surface Area and Volume Formulas
Sphere: SA=4πr2, V=34πr3; cube: SA=6s2, V=s3; rectangular solid: SA=2lh+2lw+2wh, V=lwh; cylinder: SA=2πr2+2πrl, V=πr2l.
Calculating SA:V Ratio
Find surface area and volume with the correct formula, then divide surface area by volume.
Exchange of Materials and SA:V
Higher ratios allow faster nutrient uptake, waste removal, and chemical exchange across membranes.
Membrane Folding and SA:V
Folds increase surface area greatly without much increase in volume.
Root Hairs
Thin extensions of root epidermal cells that increase surface area for water and mineral absorption.
Guard Cells and Stomata
Paired cells regulate stomatal openings, controlling gas exchange and water loss in leaves.
Gut Epithelial Cells and Microvilli
Intestinal lining cells have membrane projections that increase surface area for nutrient absorption.
Cilia
Short hairlike cell projections that increase exposed surface and help move materials past cells.
Heat Exchange and Body Size
Smaller bodies lose heat faster because they have more surface area relative to volume.
Metabolic Rate per Unit Body Mass
Smaller multicellular organisms usually use energy faster per gram than larger organisms.
Cell Size Limits and Plasma Membrane Exchange
As cells grow, volume increases faster than membrane area, limiting efficient material exchange.
Phospholipid Bilayer
A double phospholipid layer with a hydrophobic interior that causes selective permeability.
Integral and Peripheral Proteins
Integral proteins penetrate the bilayer; peripheral proteins attach loosely to the membrane surface.
Cholesterol
A steroid in animal membranes that stabilizes the bilayer and buffers fluidity changes.
Fluid Mosaic Model
A dynamic membrane model with moving phospholipids and embedded proteins forming a flexible mosaic.
Factors Affecting Membrane Fluidity
Higher temperature and unsaturated tails increase fluidity; cholesterol buffers fluidity across temperatures.
Selective Permeability
A membrane property where small nonpolar molecules cross easily but polar molecules and ions do not.
Transport Proteins and Channel Proteins
Embedded membrane proteins that move hydrophilic substances across the bilayer; channels are passive, transport proteins may actively pump.
Amphipathic Phospholipids
Phospholipids have hydrophilic phosphate heads and hydrophobic fatty acid tails.
Membrane Proteins
Embedded or attached proteins have hydrophobic regions in the bilayer and hydrophilic regions exposed to water.
Glycoproteins, Glycolipids, and Glycocalyx
Carbohydrate-tagged membrane molecules form an outer coat used for recognition, adhesion, and protection.
Unsaturated Fatty Acids
Fatty acid tails with double bonds create kinks that increase membrane fluidity.
Saturated Fatty Acids
Straight fatty acid tails pack tightly together and decrease membrane fluidity.
Cell Wall
A rigid outer layer that supports cells, limits some substances, and prevents osmotic lysis.
Selective Permeability
A membrane property that allows some substances to cross more easily than others.
Concentration Gradient
A difference in the concentration of a substance across space or across a biological membrane.
Osmosis
The diffusion of water across a selectively permeable membrane toward the region with a higher solute concentration.
Passive Transport
The net movement of substances down their concentration gradient without direct energy input.
Active Transport
The movement of substances across a membrane using cellular energy, often against their concentration gradient.
Transport Proteins
Membrane proteins that move specific substances across the bilayer through channels, carriers, or pumps.
Aquaporins
Channel proteins that allow rapid movement of water across cell membranes.
Sodium-Potassium Pump
An ATP-powered membrane pump that exports Na+ and imports K+ against their concentration gradients.
Membrane Permeability by Molecule Type
Small nonpolar molecules cross freely; water crosses slowly or via aquaporins; large polar molecules and ions require transport proteins.
Cell Wall and Membrane Transport
A rigid outer layer that provides structural support and filtering, while the plasma membrane serves as the primary selective barrier.
Diffusion
The passive movement of particles from an area of high concentration to an area of low concentration.
Endocytosis and Exocytosis
Energy-requiring vesicle transport processes that move materials into cells or out of cells.
Tonicity
The relative solute concentration of a solution that determines the direction and extent of water movement across a membrane.
Facilitated Diffusion
Passive movement of polar molecules or ions through membrane proteins down their concentration gradient.
Large Polar Molecules and Membrane Passage
Substances that cannot pass easily through the hydrophobic bilayer directly and must move through transport proteins.
Concentration Gradient
A difference in substance concentration across space that drives net movement from high to low concentration.
Membrane Polarization
An unequal distribution of electrical charge across a membrane caused by ion movement.
Aquaporins
Channel proteins that allow rapid movement of large quantities of water across membranes.
Simple Diffusion vs. Facilitated Diffusion
One process crosses the bilayer directly while the other uses transport proteins, though both move substances down gradients without energy input.
Channel Proteins and Ion Passage
Membrane proteins forming hydrophilic pathways that let charged particles cross the hydrophobic bilayer.
Passive Transport
Movement of substances across membranes down their concentration gradient without direct energy input.
Hypotonic, Hypertonic, and Isotonic Solutions
Hypotonic solutions have lower solute concentration, hypertonic solutions have higher solute concentration, and isotonic solutions have equal solute concentration relative to another solution.
Osmosis
The passive diffusion of water across a selectively permeable membrane from higher to lower water potential.
Solute Potential
The component of water potential that reflects the effect of dissolved solutes, which is always zero or negative.
Solute Potential Equation
The equation Ψs=−iCRT, where i is the ionization constant, C is molar concentration, R is the pressure constant, and T is temperature in Kelvin.
Osmolarity
The total concentration of dissolved solute particles in a solution.
Concentration Gradient
A difference in substance concentration across space that drives net movement from high to low concentration.
Osmoregulation
The control of internal water balance and solute concentration by an organism to maintain homeostasis.
Contractile Vacuole
An organelle in many freshwater protists that pumps out excess water entering the cell by osmosis.
Central Vacuole
A large organelle in plant cells that stores water and helps maintain turgor pressure.
Plasmolysis
The shrinking of a plant cell membrane away from the cell wall due to water loss in a hypertonic environment.
Lysis and Crenation
Lysis is cell bursting from excessive water gain, whereas crenation is cell shriveling from water loss in animal cells.
Water Potential
The potential energy of water per unit volume, represented by the equation Ψ=Ψ<em>p+Ψ</em>s.
Pressure Potential and Turgor Pressure
Pressure potential is physical pressure that raises water potential, while turgor pressure is the outward hydrostatic pressure against a plant cell wall.
Water Potential Gradient
The path along which water passively moves, from areas of higher water potential to lower water potential.