Pre-Lab Quiz on lacZ CRISPR Gene Editing Procedures
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10 Terms
_____________________ are sequences in the genomes of some prokaryotes that act as a genomic record of previous viral attack.
Clustered regularly interspaced palindromic repeats
2 multiple choice options
T/F: Transformation solution can be kept at room temperature for this lab.
False
1 multiple choice option
Match the component of the CRISPR-Cas9 system to the definition that best describes them.
a bacterial endonuclease that forms a double-strand break (cuts) DNA at a specific site within a larger recognition sequence, or target site.
Cas9 enzyme
3 multiple choice options
Match the component of the CRISPR-Cas9 system to the definition that best describes them.
an engineered form of guide RNA that forms a complex with Cas9.
sgRNA
3 multiple choice options
Match the component of the CRISPR-Cas9 system to the definition that best describes them.
a sequence motif immediately downstream of the protospacer sequence in the Cas9 recognition sequence that is required for Cas9 function.
PAM
3 multiple choice options
Match the component of the CRISPR-Cas9 system to the definition that best describes them.
part of the CRISPR RNA or crRNA in nature, a typically 20-nucleotide region that is complementary to the target DNA sequence and that defines where Cas9 cuts.
guiding region
3 multiple choice options
Match the component of the CRISPR-Cas9 system to the definition that best describes them.
called the transactivating CRISPR RNA or tracrRNA in nature, a region that forms a multi-hairpin loop structure (scaffold) that binds tightly in a crevice of the Cas9 protein.
scaffolding region
3 multiple choice options
To use CRISPR Cas-9 scientist must first identify the _________ in the human genome that is causing the health problem.
sequence
Once a particular stretch of nucleotides has been identified the scientist must then create a ___________.
guide RNA
3 multiple choice options
T/F: CRISPR/Cas9 is the cheapest and easiest of the gene editing tools.
True
1 multiple choice option