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Discard at least three times the line volume
Indwelling lines commonly contain heparin; before drawing a sample you should:
Larger molecules and formed elements
Hemoconcentration refers to an increased concentration of:
Tourniquet application longer than 1 minute
A primary cause of hemoconcentration during phlebotomy is:
AST, total proteins, lipids, cholesterol, and iron
Prolonged tourniquet time tends to significantly increase measured:
Larger nonfilterable molecules (e.g., enzymes, proteins, lipids)
Postural changes from supine to seated can increase concentrations of:
Administration, distribution, metabolism, and elimination
For therapeutic drug monitoring, timing of collection must consider:
Patient has extensive burns
Capillary puncture is preferred when:
2–3 mm adults; 1.6 mm infants
Typical skin puncture depths are approximately:
Fingertip pad (lateral sides) of middle or ring finger
Appropriate adult fingerstick sites include:
Heel (medial or lateral plantar surface within safe zone)
For infants, the recommended capillary site is:
Wiped away to reduce tissue fluid contamination
During fingerstick collection, the first drop of blood is typically:
Lab coat or gown and hand hygiene
Universal precautions require gloves and:
Dispose immediately in a sharps container without recapping
After removing a needle from a patient, you should:
Fresh 10% bleach solution
Blood spill cleanup typically uses:
Squeeze site, wash with soap and water, document and follow protocol
After an accidental needlestick, the first actions include:
About 79% effectiveness in preventing seroconversion
Post‑exposure prophylaxis with zidovudine (AZT/ZDV) after HIV exposure has shown:
Away from patients, especially children and psychiatric patients
To protect patients, phlebotomy equipment should be kept:
Artery; apply firm pressure for >5 minutes
If bright red pulsatile blood is obtained during attempted venipuncture, you likely hit an:
Move the needle slightly forward to enter the lumen
For troubleshooting an incomplete collection, a first maneuver is to:
Adjust angle slightly to center in lumen
If the bevel is against the vein wall, a corrective step is to:
Prevent additive carryover contamination
Order of draw (venous, per Henry mnemonic YBGGLG) helps to:
Prioritize tests most sensitive to tissue fluid and platelet activation
In capillary draws (per Rodak), the order differs primarily to:
Crossmatch, chemistry, immunology/serology after clotting
A plain red‑top tube (no additive) is commonly used for:
Separate serum via gel barrier after clotting
A gold‑top SST is designed to:
Plasma chemistries with gel separation
A light green PST with lithium heparin is primarily for:
Contain no contaminating metals for zinc, copper, lead
A dark blue (royal blue) trace‑element tube is designed to:
Preserve glucose by inhibiting glycolysis
A light gray‑top tube contains sodium fluoride/potassium oxalate primarily to:
HLA typing, paternity testing, DNA studies
A yellow‑top ACD (acid‑citrate‑dextrose) tube is used for:
Microbiology culture of aerobes/anaerobes/fungi
A yellow‑black (culture) tube containing broth is used for:
Westergren ESR (erythrocyte sedimentation rate)
A black‑top tube with buffered sodium citrate is used for:
STAT serum chemistries needing rapid clotting
An orange‑top tube containing thrombin is best for:
Serum lead determination
A light brown‑top tube with sodium heparin (low lead) is used for:
Immunohematology (blood bank)
A pink‑top tube with K2/K3 EDTA is intended for:
Molecular/PCR and DNA testing
A white‑top tube with potassium EDTA is primarily for:
Protection (immune and hemostasis)
The major functional categories of blood include transport, regulation, and: