Week 1 - DNA Replication
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17 Terms
Meselson & Stahl - Process
Grow E coli in N15 medium
Transfer to N14
CsCl centrifugation - days
Isotopes settle into bands
Meselson & Stahl - Findings
1st round - intermediate
2nd round - intermediate + light
Semiconservative
9 Step Process - Prokaryotes
ORI
Bubble + fork
Helicase
SSBP
RNA primers - primase
DNA polymerase III
Topoisomerase
Remove RNA primers - DNA polymerase I
DNA ligase
9 Step Process - Eukaryotes
ORI
Bubble + fork
Helicase
SSBP
RNA primers - DNA polymerase a
Pol e + d
Topoisomerase
Remove RNA primers - nucleases
DNA ligase
SSB Proteins
Single strand binding
Hold strands apart
SSBP Units - Pro vs Eu
1 vs 3
Leading vs Lagging Synthesis - Eu
Polymerase epsilon vs delta
Topoisomerase
Prevent tangling
Relieve tension
Temporary breaks
DNA polymerase I - Prokaryotes
Remove RNA primers + replace with DNA
Telomeres
Repeated non coding DNA sequences at end of chromosomes
Protective buffer
End Replication Problem
Telomere shortens each replication
Hayflick limit - 70
Cancer Cells
Telomeres DO NOT shorten
Produce telomerase
Telomerase
Enzyme adds telomeres using RNA template
Cancer/stem/germ cells
Models - Pro
Theta
Rolling circle
Model - Eu
Linear
Euchromatin
Loosely packed
Heterochromatin
Condensed