qualitative elisa

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18 Terms

1
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step 1

enter

2
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step 2

label 12 wells (+++ —- sss)

3
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step 3

50 ul AG into each well

4
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step 4

2 min incubate

5
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step 5

wash protocol

6
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wash protocol

tip wells upside down, discard wet towers, use transfer pipet to fill well with wash buffer

7
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step 6

do step 5 again

8
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step 7

50 ul of + into + wells

9
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step 8

50 ul of - into - wells

10
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step 9

50 ul of s into s wells

11
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step 10

incubate 2 min

12
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step 11

wash 2 timess

13
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step 12

50 ul ELA into each well

14
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step 13

incubate 2 min

15
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step 14

wash (step 5) 3 times

16
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step 15

50 ul SUB into each well

17
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step 16

clean up: dispose tips, disinfect, PPE, wash hand

18
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step 17

observe and report results