Microscopes

Anton Von Leeuwenhoek

First to observe living bacteria and draw them

Also looked at protists, sperm and blood

Leeuwenhoek used a

simple scope

Simple scopes are

magnifying glass (hand lens)

Magnifying Glass magnification

3-40x

Robert Hooke

Used compound scope to examine cork

Coined the term cell. Saw Dead plant cells

Compound Light Microscope is

Most commonly used microscope

Compound Light Microscope uses

light and lenses to magnify & view the specimen

Compound light microscope number of lenses

Two, Ocular and Objective

Ocular Lense

The eyepiece

Objective Lense

near the object being viewed

Light Microscope total magnification

Ocular (10X) x Objective (40x)

Our Light microscope total magnification

40 - 1000x

Eyepiece (Ocular Lens)

The part you look through; usually magnifies 10x.

Objective Lenses

The parts that do the magnifying. You start with the low power objective

Revolving Nosepiece

Holds all the objective lenses; rotate it to switch between objectives.

Stage

The flat platform where the slide is placed for viewing.

Mechanical Stage

The platform where you put your slide. It is moved by using the knobs below the stage.

Stage Clips

Hold the slide in place on the stage.

Coarse Adjustment Knob

Used for focusing under low power by moving the stage up and down.

Fine Adjustment Knob

Used for fine focusing after using the coarse adjustment

Light Source (Illuminator)

Provides light to pass through the specimen; brightness can be adjusted with the diaphragm.

Diaphragm

Controls the amount of light passing through the specimen.

Base

The bottom part of the microscope; use one hand under the base when carrying.

Arm

Used for carrying the microscope; hold one hand on the arm when transporting.

Body Tube

Connects the eyepiece to the objective lenses.