Microbiology Chapter 3 Bio-Rad

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Prokaryote

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51 Terms

1

Prokaryote

A unicellular organism that lacks a nucleus and membrane bound organelles

<p>A unicellular organism that lacks a nucleus and membrane bound organelles</p>
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2

Eukaryote

A cell that contains a nucleus and membrane bound organelles

<p>A cell that contains a nucleus and membrane bound organelles</p>
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3

gram positive

purple

<p>purple</p>
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4

gram negative

pink

<p>pink</p>
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5

Alexander Fleming

Discovered penicillin in 1928

<p>Discovered penicillin in 1928</p>
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6

Kirby-Bauer Test

The test to determine the efficacy of antibiotics or the antibiotic resistance of bacteria. Zone of Inhibition

<p>The test to determine the efficacy of antibiotics or the antibiotic resistance of bacteria. Zone of Inhibition</p>
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7

Pencillin

antibiotic

<p>antibiotic</p>
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8

petri dish

a shallow dish used to culture bacteria

<p>a shallow dish used to culture bacteria</p>
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9

aseptic

preventing infection; having a cleansing effect

<p>preventing infection; having a cleansing effect</p>
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10

Pathogen

a bacterium, virus, or other microorganism that can cause disease.

<p>a bacterium, virus, or other microorganism that can cause disease.</p>
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11

Black Plague/Bubonic Plague

the disease that killed a large part of the European population in the Medieval times

<p>the disease that killed a large part of the European population in the Medieval times</p>
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12

Archeabacteria

ancient bacteria; live in extreme conditions

<p>ancient bacteria; live in extreme conditions</p>
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13

Eubacteria

A kingdom that contains all prokaryotes except archaebacteria

<p>A kingdom that contains all prokaryotes except archaebacteria</p>
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14

Bacillis

rod shaped

<p>rod shaped</p>
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15

Spirrilum

spiral shaped bacteria

<p>spiral shaped bacteria</p>
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16

Cocci

sphere shaped bacteria

<p>sphere shaped bacteria</p>
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17

Inoculation Loop

a metal rod with a small wire loop at the end, used as a swab

<p>a metal rod with a small wire loop at the end, used as a swab</p>
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18

centrifuge tube

glass or plastic tube that holds samples within a centrifuge

<p>glass or plastic tube that holds samples within a centrifuge</p>
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19

conical tube

Tubes ideal for cell centrifugation.

<p>Tubes ideal for cell centrifugation.</p>
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20

Bunsen burner

used to heat substances

<p>used to heat substances</p>
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21

spectrophotometer

An instrument that measures the proportions of light of different wavelengths absorbed and transmitted by a pigment solution.

<p>An instrument that measures the proportions of light of different wavelengths absorbed and transmitted by a pigment solution.</p>
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22

CFU

colony forming unit

<p>colony forming unit</p>
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23

streaking for isolation

the purpose of this procedure was to obtain isolated colonies

<p>the purpose of this procedure was to obtain isolated colonies</p>
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24

serial dilution

the process of diluting a sample several times

<p>the process of diluting a sample several times</p>
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25

Optical Density (OD)

A numerical representation of the film's ability to transmit light. Measure how cloudy a concentration is. Determine cell concentration.

<p>A numerical representation of the film's ability to transmit light. Measure how cloudy a concentration is. Determine cell concentration.</p>
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26

Transmitance

Amount of light sent through the sample

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27

Absorbance

The amount of light absorbed by a sample

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28

Koch's Postulates

series of guidelines used to identify the microorganism that causes a specific disease

<p>series of guidelines used to identify the microorganism that causes a specific disease</p>
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29

Koch's second postulate

The suspected pathogen must be grown in pure culture

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30

Koch's first postulate

The same pathogen must be present in every case of the disease

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31

Koch's third postulate

The same disease must result when the isolated microorganism is inoculated into a healthy host

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32

Koch's fourth postulate

The organism should be reisolated and shown to be the same as the original

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33

Agar

Gelatin-type material derived from seaweed

<p>Gelatin-type material derived from seaweed</p>
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34

Luria Broth (LB)

a nutritionally rich medium, is primarily used for the growth of bacteria

<p>a nutritionally rich medium, is primarily used for the growth of bacteria</p>
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35

Louis Pasteur (1822-1895)

Developed a vaccine for rabies & anthrax.

<p>Developed a vaccine for rabies & anthrax.</p>
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36

microorganism

an organism that is microscopic in size, may exist as single cells or colony of cells (aka microbe)

<p>an organism that is microscopic in size, may exist as single cells or colony of cells (aka microbe)</p>
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37

Koch's Postulates

determine if microorganisms cause disease

<p>determine if microorganisms cause disease</p>
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38

Peptidoglycan

the compounds that build cell walls in gram positive and negative cell walls.

<p>the compounds that build cell walls in gram positive and negative cell walls.</p>
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39

anaerobic

without oxygen

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40

aerobic

occurring only in the presence of oxygen.

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41

Microbiology

the study of microbes

<p>the study of microbes</p>
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42

Biosafety cabinet

Enclosed, ventilated lab workspace

<p>Enclosed, ventilated lab workspace</p>
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43

Thermophiles

Type of bacteria found at Yellowstone National Park

<p>Type of bacteria found at Yellowstone National Park</p>
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44

Insulin

A medicine created using genetically modified bacteria.

<p>A medicine created using genetically modified bacteria.</p>
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45

Conjunction

Bacteria sexual reproduction

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46

Eubacteria

true bacteria

<p>true bacteria</p>
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47

Archaebacteria

considered ancient life forms that evolved separately from bacteria and blue-green algae

<p>considered ancient life forms that evolved separately from bacteria and blue-green algae</p>
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48

Ribosomes

Makes proteins

<p>Makes proteins</p>
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49

Plasmid

small, circular piece of DNA located in the cytoplasm of many bacteria

<p>small, circular piece of DNA located in the cytoplasm of many bacteria</p>
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50

Pili

short, hairlike protein structures on the surface of some bacteria

<p>short, hairlike protein structures on the surface of some bacteria</p>
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51

Flagella

A long, whip-like filament that helps in cell motility. Many bacteria are flagellated, and sperm are flagellated.

<p>A long, whip-like filament that helps in cell motility. Many bacteria are flagellated, and sperm are flagellated.</p>
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