Purifying the DNA and Organic DNA Extraction Method

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9 Terms

1
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The importance of RNAse treatment

Taking care of RNA. Need to get rid of RNA when extracting DNA.

2
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The importance of TE buffer (3)

  • End at elusion stage

  • PCR, qPCR dilute so it can be stored

  • Primers

3
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Biphasic

  • Biphasic - two distinct phases or stages

  • Associated with organic methods

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DNA extraction methods Organic Steps (4)

  1. Lysis

  2. Acidification

  3. Extraction

  4. DNA Precipitation

Uses organic chemicals phenol/ chloroform

<ol><li><p>Lysis</p></li><li><p>Acidification</p></li><li><p>Extraction</p></li><li><p>DNA Precipitation</p></li></ol><p>Uses organic chemicals phenol/ chloroform</p>
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Lysis Organic

NaOH,SDS

<p>NaOH,SDS</p>
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Acidification organic

acetic acid, salt

<p>acetic acid, salt</p>
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Extraction

phenol, chloroform

<p>phenol, chloroform</p>
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DNA precipitation

Ethanol

<p>Ethanol</p>
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Notes on Organic Methods

  1. Phenol/ chloroform

  2. Fast and cheap

  3. Hazard

  4. Chance of Contamination