IB Bio 3.5 Genetic Modification

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PCR

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Biology

24 Terms

1

PCR

Polymerase Chain Reaction

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2

Gel Electrophoresis

Procedure used to separate and analyze DNA fragments based on their size

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3

Agarose Gel

A jelly-like slab used to separate DNA molecules on the basis of size

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4

Replication

Copying DNA

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5

Taq Polymerase

A DNA synthesis enzyme that can withstand the temperatures of PCR

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6

Kilobases

A thousand DNA nucleotide bases

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7

Genus

A group of similar species

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8

Restriction Endonucleases

An enzyme that cuts DNA at specific sites

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9

Paternity

Father

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10

DNA Profile/Fingerprint

A pattern of bands made up of DNA fragments

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11

Plasmid

A small ring of DNA in bacteria for the transfer of useful genes between bacteria

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12

DNA Ligase

Enzyme joins DNA backbone together sealing any cuts

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13

Sticky Ends

Single stranded ends of DNA left after cutting with enzymes

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14

Gene Transfer

Movement of genetic information between organisms

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15

Reverse Transcriptase

An enzyme encoded by some certain retroviruses that uses RNA as a template for DNA synthesis

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16

Clones

Identical genetic copies, monozygotic twins, budding, plantlets, bulbs

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17

Zygote

Fertilized egg

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18

Budding

A form of asexual reproduction of yeast and hydra in which a new cell grows out of the body of a parent

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19

Bulbs

Genetically identical cells with food stored ready to produce a genetically identical parent plant

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20

Transgenic

An organism that contains genes from another organism

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21

Vector

A method for carrying DNA from one cell to another cell

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22
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23

Species

A group of similar organisms that can be breed and produce fertile offspring

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24

Maternity

Mother

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