Steps for ELISA Test

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9 Terms

1
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Step 1 for ELISA Test

Add in antigens and other proteins in the plastic walls.

2
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Step 2 for ELISA Test

Wash out unbound proteins with buffer.

3
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Step 3 for ELISA Test

Add in primary antibody.

4
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Step 4 for ELISA Test

Wash out any primary antibodies that did not stick.

5
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Step 5 for ELISA Test

Add in enzyme-linked secondary antibody.

6
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Step 6 for ELISA Test

Enzyme-linked secondary antibody attaches to primary antibody.

7
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Step 7for ELISA Test

Wash out (with buffer) unbound secondary antibodies.

8
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Step 8 for ELISA Test

Add in enzyme substrate.

9
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Step 9 for ELISA Test

Enzymes work on substrate causing substrate to oxidize (color change).