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What is sub cellular fractionation protocol procedure?
It is a procedure for separating nuclear, membrane and cytoplasmic cell fractions using centrifugation methods (fractionated ultracentrifugation)
What is FRAP?
Fluorescence recovery after photobleaching
relies on a short and intense laser illumination of fluorophores in a confined area of the cell to locally photodestruct the fluorophores
What are the three types of lipids present in the PM?
Phospholipids (70%)
Cholesterol (29%)
Glycolipids (10%)
What needs to happen for us to have a phospholipid?
Only when there is a modification of phosphate group by an alcohol
