FAULT/PROBLEMS OBSERVED - REMEDY

studied byStudied by 0 people
0.0(0)
learn
LearnA personalized and smart learning plan
exam
Practice TestTake a test on your terms and definitions
spaced repetition
Spaced RepetitionScientifically backed study method
heart puzzle
Matching GameHow quick can you match all your cards?
flashcards
FlashcardsStudy terms and definitions

1 / 56

encourage image

There's no tags or description

Looks like no one added any tags here yet for you.

57 Terms

1

Tissue may be softened by soaking in a small dish or bowl containing detergent, phenol or Molliflex.

Brittle or hard tissue

New cards
2

Repeat dehydration with absolute alcohol then clear again.

Clearing agent turns milky as soon as tissue is placed on it.

New cards
3

Block is trimmed down to nearest to the tissue. Remaining wax is melted on embedding oven and paraffin impregnation is repeated, changing the paraffin at least once before blocking.

On trimming, tissue smells of clearing agent.

New cards
4

Repeat clearing; if object has already been embedded, prolong clearing up to 12 hours, then re-embed.

Tissue is opaque, section-cutting is difficult due to the presence of alcohol.

New cards
5

Repeat the whole procedure.

Tissue shrinks away from wax when trimmed.

New cards
6

Repeat fixation

Tissue is soft when block is trimmed.

New cards
7

Repeat impregnation

Airholes found on tissue during trimming.

New cards
8

Re-embed in freshly filtered wax.

On trimming, wax appears crystalline.

New cards
9

Repeat paraffin impregnation, then re-embed.

Paraffin block after cooling is moist and crumbles.

New cards
10

Re-trim the block.

Sections fail to form edges of the block ribbons due to surfaces and edges of the block is not parallel.

New cards
11

Re-adjust and re-orient the block.

Sections fail to form edges of the block ribbons due to horizontal surface of the block is not parallel to the knife.

New cards
12

Coat horizontal edges of the block with wax of lower melting point.

Sections fail to form edges of the block ribbons due to paraffin wax is too hard.

New cards
13

Reduce the tilt.

Sections fail to form edges of the block ribbons due to knife is tilted too much.

New cards
14

Readjust the thickness of the sections.

Sections fail to form edges of the block ribbons due to sections are too thick.

New cards
15

Hone and strop.

Sections fail to form edges of the block ribbons due to knife is dull.

New cards
16

Sharpen the knife.

Sections roll up on cutting so that they adhere and get broken against the knife edge due to knife is blunt.

New cards
17

Reduce the tilt

Sections roll up on cutting so that they adhere and get broken against the knife edge due to the tilt of knife is too great.

New cards
18

Clean the knife edge.

Sections roll up on cutting so that they adhere and get broken against the knife edge due to the knife edge is dirty.

New cards
19

Adjust the knife so that knife edge will present a uniformly sharp edge to the block, or sharpen.

Ribbon is curved, crooked or uneven instead of straight due to blunt or dull spot on the knife, producing an irregular knife edge.

New cards
20

Re-trim the block.

Ribbon is curved, crooked or uneven instead of straight due to edges of the block are not parallel but round or wedge shaped.

New cards
21

Readjust the knife and block.

Ribbon is curved, crooked or uneven instead of straight due to the knife is not parallel to the block.

New cards
22

Re-sharpen the knife.

Sections are compressed, wrinkled or jammed due to knife is blunt or dull.

New cards
23

Cool the block on ice water until firm.

Sections are compressed, wrinkled or jammed due to paraffin block is warm and soft.

New cards
24

Clean the knife edge.

Sections are compressed, wrinkled or jammed due to knife edge is coated with paraffin.

New cards
25

Readjust thickness of the section.

Sections are compressed, wrinkled or jammed due to sections are too thin.

New cards
26

Tighten the screw.

Sections are compressed, wrinkled or jammed due to microtome set screw is loose.

New cards
27

Reduce the tilt.

Sections are compressed, wrinkled or jammed due to tilt of knife is too vertical.

New cards
28

Re-sharpen, using a knife back or automatic knife sharpener.

Sections are squashed (width of each section is less than that of the block) due to the bevel of knife is lost due to incorrect sharpening.

New cards
29

Re-embed in freshly filtered wax if necessary.

A hole is formed in the section due to bubble or dirt formed in the embedding medium.

New cards
30

Re-process tissue.

A hole is formed in the section due to tissue is not processed properly and will not form a section (especially if center is raw).

New cards
31

Re-process tissue

A hole is formed in the section due to under-processed portion of tissue bursts on contact with warm water.

New cards
32

Once embedded in paraffin wax, decalcification is impractical; use a base-sledge microtome with a wedge knife.

A hole is formed in the section due to hard spot in tissue due to calcium.

New cards
33

Tighten the screw

Sections of unequal thickness are produced due to clamp set screw on knife or block holder is loose.

New cards
34

Cut blocks into smaller fragments.

Sections of unequal thickness are produced due to blocks are too large.

New cards
35

Soften the block in detergent or phenol.

Sections of unequal thickness are produced due to blocks are too hard.

New cards
36

Reduce the tilt.

Sections of unequal thickness are produced due to tilt of knife is too great or bevel is not cleared, hence object is compressed against the knife edge.

New cards
37

Breathe out or blow gently on the block and knife to break up static electricity, or boil water in the room to increase humidity.

Sections adhere to the knife or other parts of the microtome because of static electricity due to low atmospheric humidity.

New cards
38

Clean the knife edge.

Sections adhere to the knife or other parts of the microtome due to knife edge is dirty.

New cards
39

Sharpen the knife.

Sections adhere to the knife or other parts of the microtome due to knife edge is dull.

New cards
40

Reduce the tilt.

Sections adhere to the knife or other parts of the microtome due to knife tilt is to great.

New cards
41

Reduce the tilt

Sections are lifted from the knife on upstrokes due to knife tilt is too great.

New cards
42

Sharpen the knife.

Sections are lifted from the knife on upstrokes due to knife is dull.

New cards
43

Cool paraffin wax in ice water.

Sections are lifted from the knife on upstrokes due to due to paraffin is too soft or room temperature is warm.

New cards
44

Increase the tilt.

Resistance is felt on the lower part of the section during cutting due to the tilt of knife is too dull; paraffin block is therefore compressed against the base of the knife towards the end of stroke.

New cards
45

Treat with phenol during processing or collodionize.

Horizontal or parallel lines or furrows across the section (“chatters”) are seen because of knife edge vibration due to hardness of tissue.

New cards
46

Reduce the tilt.

Horizontal or parallel lines or furrows across the section (“chatters”) are seen due to tilt of knife is too great.

New cards
47

Sharpen the knife.

Section cut is sometimes thin, sometimes thick due to knife is blunt.

New cards
48

Adjust the knife so that the knife edge will present a uniformly sharp edge to the block, or sharpen.

Section cut is sometimes thin, sometimes thick due to knife is not clamped properly.

New cards
49

Tighten adjusting and locking screws.

Section cut is sometimes thin, sometimes thick due to knife or block holder is loose.

New cards
50

Increase the tilt.

Section cut is sometimes thin, sometimes thick due to knife tilt is too small that block is compressed by bevel and section is not cut.

New cards
51

Readjust the tilt.

Knife makes a hard metallic scraping or ringing sound on backstroke, when section is cut due to tilt of knife is too slanted or too big.

New cards
52

Take fresh block treated with phenol during processing.

Knife makes a hard metallic scraping or ringing sound on backstroke, when section is cut due to tissue is too hard.

New cards
53

Change the knife.

Knife makes a hard metallic scraping or ringing sound on backstroke, when section is cut due to knife blade is too thin.

New cards
54

Refreeze the tissue block.

Frozen tissue crumbles and comes off the block holder when cut due to freezing is not adequate.

New cards
55

Warm the tissue with fingers.

Frozen tissue chips into fragments when cut due to tissue is frozen too much.

New cards
56

Adjust the block holder to make the block edges parallel to the knife.

Ribbons are crooked because the top and bottom edges of block are not parallel to edge of blade/sides of block are not perpendicular to the blade.

New cards
57

Microtome needs recalibration.

Sections are too thick due to wrong micrometer setting.

New cards

Explore top notes

note Note
studied byStudied by 17 people
312 days ago
5.0(1)
note Note
studied byStudied by 38 people
379 days ago
5.0(1)
note Note
studied byStudied by 21 people
759 days ago
5.0(1)
note Note
studied byStudied by 56 people
798 days ago
5.0(4)
note Note
studied byStudied by 411 people
345 days ago
5.0(2)
note Note
studied byStudied by 20 people
898 days ago
5.0(1)
note Note
studied byStudied by 14 people
899 days ago
5.0(1)

Explore top flashcards

flashcards Flashcard (147)
studied byStudied by 56 people
850 days ago
5.0(1)
flashcards Flashcard (31)
studied byStudied by 16 people
87 days ago
5.0(1)
flashcards Flashcard (90)
studied byStudied by 26 people
422 days ago
5.0(2)
flashcards Flashcard (46)
studied byStudied by 5 people
395 days ago
5.0(1)
flashcards Flashcard (297)
studied byStudied by 602 people
832 days ago
5.0(2)
flashcards Flashcard (26)
studied byStudied by 3 people
815 days ago
5.0(1)
flashcards Flashcard (42)
studied byStudied by 11 people
548 days ago
5.0(1)
flashcards Flashcard (38)
studied byStudied by 7 people
638 days ago
5.0(1)
robot