HPB

diamond in my mouth when i grin

table-top balance

table-top balance

• 0.001 g-300g

• Weigh boat/paper is needed

• 1mL= 1 g

• last decimal is estimated

analytical balance

• One in prep room

• Density- 0.0001 g, max- 65g

• C-102 has each in orange yellow red and pink lab stations

• C-104 has one in the yellow lab station

Microcentrifuge Tube Holder/Rack

• Has 80 count

4-way tube holder/rack

• aka flipper rack

• holds 50 ml conical tubes, 15 ml conical/test tubes, microcentrifuge tubes, mini centrifuge tubes

Drying Rack/Peg Rack

• Holds 15 ml conical, small test tubes

• Good for air drying/holding tubes (use w/ towel underneath)

Serological Pipettors

• Used w/ serological pipettes

• Red: 25 ml

• Green: 10/5 ml

• Blue 1-2 ml

Bactoincinerator/Microincinerator

• Sterilize metal

• Slide dryer is used to air dry slides quicker

• Bacteriological work- use on high

DPTP

• Above yellow stations sink for both C-104 and C-102

• Sterile: under lab supplies

Spatula/Scoopula

• Used to remove solid reagents from their containers

• Must wash and dry before and after each use

Heat Block

• Heats tubes of various sizes: one of the holes is where a thermometer needs to be added

• NOT the same as hot plate

Inoculating Loop

• Transfer and streak bacteria into/onto culture medium

• Needs to be sterilized with bactoincinerator 

Beakers

• NOT FOR MEASURING 

• 1-100 mL 

• 1- 600 or 400 mL 

• ½- 250 mL 

• 1- 150 or 100 mL 

• 1-50 mL   

Erlenmeyer Flask

• NOT FOR MEASURING 

• 1-250 mL 

• 2-125 mL 

• 1-500 mL 

pH indicator paper/strip

• 0-14 = full range

• 5-10 = bases

• 0-6 = acids

Glass Stirring Rod

• 2 straight and 1 bent (bent is not a stirring rod)

• in white tray

Laboratory Label Tape

• each drawer- 2

• large and small width

• ⅔ index finger 

• only write with sharpie markers

  
  

Balance Brush

• always needed

• take out the balance, take out the brush

Autoclave

• stripes change from white to black in high temperature/pressure autoclave cycle

• in prep room under the sink

Vortexer

• mixing solutions in conical tubes or microtubes

• secure the lid*

Microtube

• come in several sizes

• 1.5-1.7 mL

Conical Tubes

• 15 mL and 50 mL above pink sink

• have graduations for measuring

Colony Counter

• place a plate of bacteria to help you count (lights up)

Ice Bucket

• 1 scoop of ice from the ice machine in the prep room

• Leave scoop on counter, never in the machine

• Close ice machine door

Micropipettes

• P-10

• P-20

• P-100 (C-102: Mrs. Landreville’s class)

• P-200 (C-104: Ms. Cherry’s class)

• P-1000 

• name = largest volume they can measure

Stirring Hot Plate

• 2 Knobs:

- Left: controls heat

- Right: controls the RPM of the magnetic stir bar

Micropipette Tips

• Sterilized in autoclave for use in experiments

• Different size tips for different sized pipettes:

- p-10: small 

- p-20, p-100, p-200: medium 

- p-1000: large

Mortar and Pestle

• Used for grinding and mixing

• Mortar is the bowl

• Pestle is the stirring tool

Test Tube Holder

• Used for placing/removing tubes in hot or really cold environments

Visible Light Spectrophotometer

• detect different types of concentrations

Graduated Cylinder w/ Collar

• collar goes at the top

• use meniscus to measure

• - 10 ml

  - 25 ml

  - 100 ml

  
  

Media Bottles

• temperature resistant

• Can be used in autoclave, microwave, fridge, freezer

Tabletop Centrifuge

• microtest tubes

• spins solutions

• ALWAYS BALANCE out where your tubes are. For example either all six tubes are in or there is a tube in every other spot