Bio 251 lab 1

ocular lens

what is 1 ( Lens located in eye piece with 15x magnification)

objective lens

what is 2 (Lenses on revolving nose piece, 4x, 10x, 40x, and 100x magnification)

arm

what is 3 (Metal area in back for holding microscope with hand)

Stage

what is 4 ( Flat surface for placing slide.)

lamp switch

what is 5 (On/Off switch for light source.)

Rheostat

what is 6 (Knob that regulates the amount of light leaving the lamp)

Mechanical stage

what is 7 (Knobs that move the platform stage from side to side and forward and backward.)

Mechanical Stage 6

what is 6 (Knobs that move the platform stage from side to side and forward and backward.)

fine adjustment knob

what is 1 and 2 (Small inner knob on both sides next to coarse adjustment knob, used for fine focus with 40x and 100x objective

Base

What is 8 (Flat metal bottom for holding microscope when carrying.)

Condenser Lens

What is 3 (concentrates the light reaching the specimen.)

Course Adjustment Knob

What is 5 (Large knob on both sides toward base, used with 4x and 10x for initial focusing.)

Iris Diaphragm

What is 4 (Lever on condenser that regulates amount of light entering specimen.)

condenser adjustment knob

What is 1 (raises and lowers the condenser)

Lamp/light source

What is 2 (provides light to illuminate the object)

60x

Calculate total magnification:

15x x 4x

150x

Calculate total magnification:

15x x 10x

600x

Calculate total magnification:

15x x 40x

1500x

Calculate total magnification:

15x x 100x

0.2 micrometers

what is the Maximum Resolution

Parfocal

A property whereby an image in focus in a lower power will remain in focus when changing to a higher power objective.

Archaea

Microorganisms that are similar to bacteria in size and simplicity of structure but radically different in molecular organization. They are now believed to constitute an ancient intermediate group between the bacteria and eukaryotes.

Bacteria

Unicellular microorganisms that have cell walls but lack organelles and an organized nucleus, including some that can cause disease.

Eukarya

Any of various single-celled or multicellular organisms of the domain Eukaryota, characterized by cells that contain a distinct membrane-bound nucleus and by the occurrence of DNA transcription inside the nucleus and protein synthesis in the cytoplasm, in contrast to prokaryotes.

Protists

Generally microscopic, growing as individual cells or occasionally in colonial form (small groupings). Eukaryotic cells with one or more nuclei and organelles. In general, they are larger and more complex than bacteria.

Animals

Worms are multicellular and are more complex in structure than archaea and bacteria, protists, or fungi. They are generally, but not always, larger in size as well.

Paramecium

What is this?

Planaria

What is this?

Turbatrx (Vinegar Eels)

What is this?

Penicillium

What is this?

Rhizopus

What is this?

Volvox

What is this?

Amoeba

What is this?

Euglena

What is this?

Saccharomyces cerevisiae

What is this?

Aspergillus

What is this?

1.That lab coat, goggles, closed-toe shoes, and hair ties (for long hair) are required in the lab.

what is the basic safety in the lab?

4.Wash hands before and after the lab session:

when should you wash your hands

Lab coat buttoned up completely when worn. Leave lab coats in the lab, stored in a plastic bag, labeled with your name.

how should your lab coat be worn and store away

Goggles are required for lab. wear goggles and

Are goggles require in lab ?

leave in the lab in a sealed plastic bag,

how are goggles store

Goggles should always be used when working with liquid cultures

when should you bee using goggles

is not know to cause disease in human to 4 that can be life threatening to human

what are the Bio safety levels (BSL) 1 - 4.

-Not known to cause disease in healthy humans

-Standard open bench microbiology

what are the health risk and practices needed for each level for BSL 1

-Associated with human disease in healthy but easily contained. Transmission through percutaneous, ingestion, mucous membrane

- BSL-1 practice plus: Limited access Biohazard warning signs, "Sharps" precautions, disposal of waste and surveillance

what are the health risk and practices needed for each level for BSL 2

-Disease may have serious or lethal consequences

--BSL-2 practice plus: controlled access

What are the health risk and practices needed for each level for Bs3

-Agents with high risk of life threatening disease

-BSL-3 practice plus

what are the health risk and practices needed for each level for Bs4

They can be found in every habitat on earth

what is Ubiquitous

special type of interaction between species. Sometimes beneficial, sometimes harmful,

what is symbiotic relationship

are microbes that inhabit our bodies in ways that may benefit both them and us. ( ex: Barnacles and Whales. The barnacles are just along for the ride. They don't harm the whales or feed on the whales as true parasites do. Barnacles don't serve any obvious advantage to the whales, but they give helpful lice a place to hang onto the whale without getting washed away by water.)

what is commensals

because when they have the opportunity to cause disease they will often do so

what is opportunists

The introduction of a pathogen or antigen into a living organism to stimulate the production of antibodies.

what is Inoculation

distinct and separated from other similar masses, it is called a

what is Colonies

Culture media can be liquid or solid at room temperature is used as a nutrient source to allow microbes to grow.

what is culture media, is it solid or liquid and what is it use for?

allow the growth of many microorganisms that do not need special growth factors.

General purpose media

contain special growth factors required by some microorganisms to grow

what is Enrichment media?

show differences between microbial colonies based on their cultural characteristics (what they look like when growing in colonies).

Differential media

is used to inhibit the growth of some groups of microorganisms and select for the growth of other microorganisms.

what is Selective media

General or all-purpose media

what culture media for TSA (Tryptic soy agar )

Selective media

what culture media for SDA ( Sabouraud's agar plate )

Enrichment and differential media

what culture media is the sheep BAP (Blood agar place)

light microscopy is commonly used by beginning microbiologists.

what is Microscopy

which is a property whereby an image in focus in a lower power will remain in focus when changing to a higher power objective.

what is parfocal design

Lens located in eye piece with 15x magnification

what is ocular

Lenses on revolving nose piece, 4x, 10x, 40x, and 100x magnification

what is objective lens

Lens system below the stage that concentrates the light reaching the specimen.

condenser

Domains: Archaea, Bacteria, and Eukarya.

what are the 3 domains. Know the characteristics of prokaryotic and eukaryotic cells. Know how to characterize each domain with each cell type (for example, bacteria are prokaryotic).