Unit 2: Chap 5-9 Multiple Choice by K

What is the primary purpose of a cage washer?

Clean & sanitize animal cages

An autoclave sterilizes equipment using:

Steam under pressure

Which piece of equipment is specifically designed to reduce airborne dust while emptying cages?

Bedding disposal station

HEPA stands for:

High-Efficiency Particulate Air

HEPA filters remove approx. what percentage of 0.3-micron particles?

99.7%

A biological safety cabinet primarily protects:

Personnel, animals, and the environment

A laminar flow hood protects:

The product or sterile material

Which type of cage system helps reduce disease transmission?

Individually ventilated cages

Which equipment is used to clean water bottles?

Bottle washer

Which environmental factor is NOT controlled by the HVAC system?

Animal nutrition

The “gold standard” for verifying autoclave sterilization is:

Biological indicator (spore test)

Typical autoclave sterilization occurs at approximately:

121 C (250 F)

Which PPE protects against burns while unloading an autoclave?

Heat-resistant gloves

Why should an autoclave door be opened slowly?

To prevent steam burns

Which device is commonly used to weigh laboratory animals?

Balance or scale

Which inhalant anesthetic is commonly used in laboratory animals?

Isoflurane

The purpose of an anesthesia scavenging system is to:

Remove waste anesthetic gas

Automatic watering systems should be:

Checked & sanitized regularly

What is the primary function of an animal rack?

Hold animal cages

Which equipment helps maintain a 12-hour light/dark cycle?

Lighting system

Before operating unfamiliar equipment, personnel should first:

Read the SOP & receive training

Which is not typically sterilized in an autoclave?

Electronic computers

Airflow in a bedding disposal station helps:

Reduce allergen exposure

What should be done if equipment appears damaged?

Report it immediately

Individually ventilated cages help improve:

Biosecurity

Which equipment would most likely contain a HEPA filter?

Biological safety cabinet

Which monitoring device measures room humidity?

Environmental monitor

Why are refrigerators monitored daily?

To ensure proper storage temperatures

Preventive maintenance helps:

Reduce equipment failures

Why is equipment calibration important?

Accuracy

Which equipment protects both personnel and research materials when handling infectious agents?

Biological safety cabinet

Which statement abt laminar flow hoods is true?

They protect sterile materials from contamination

Which of the following should be checked before using an anethesia machine?

Oxygen supply

Which practice helps prevent contamination during cage changing?

Wearing appropriate PPE

Which piece of equipment is least likely to require routine maintenance documentation?

Disposable gloves

Which of the following is an example of emergency equipment?

Fire extinguisher

Air pressure differences within an animal facility help:

Control contamination

Which statement is true regarding biological indicators?

They verify sterilization effectiveness

Which action best supports animal welfare and personnel safety?

Reporting malfunctioning equipment immediately

Which combination correctly matches the equipment with its primary purpose?

Autoclave—Sterilization

The primary purpose of an animal cage is to:

Provide a safe & healthy environment

The most common type of rodent cage is the:

Shoebox cage

Which cage system uses passive airflow?

Static microisolater cage

An individually ventilated cage (IVC) provides:

Active HEPA-filtered airflow

Bedding should be:

Clean, absorbent, & non-toxic

The wire bar lid on a rodent cage primarily holds the:

Food hopper

Cage cards are used to identify:

Animal info & protocol details

During cage changes, technicians should:

Observe each animal’s condition

Environmental enrichment is intended to:

Encourage natural behaviors

Which is an example of enrichment?

Nesting material

Cage changing primarily reduces:

Ammonia buildup

A leaking water bottle should be:

Replaced or repaired immediately

Overcrowding may cause:

Aggression & increased ammonia

Cage cards should always be:

Accurate & current

The purpose of bedding includes:

Absorbing urine & providing comfort

Damaged cages should:

Be removed from services

Automatic watering systems should be:

Checked regularly

Cage sanitation helps:

Reduce disease transmission

Which item provides insulation and nesting opportunities?

Bedding

A technician notices a mouse with rough hair coat & decreased activity during a cage change. The technician should:

Report the observation according to SOP

Which housing system generally provides the greatest protection against airborne contamination?

IVC

Social species should generally be:

Group housed when compatible

Why should cage cards always remain with the correct cage?

To maintain accurate animal identification

Which is an example of biosecurity?

Following clean-to-dirty work flow

Which bedding characteristic is undesirable?

Contaminated

Animals should be observed:

During routine husbandry procedures

Which gas commonly increases if cages are not changed regularly?

Ammonia

The primary purpose of enrichment is:

Improve welfare

Which item is not part of biosecurity?

Cross-contamination

A filter top on a static cage primarily:

Provides passive protection from contamination

Before placing clean cages on a rack, they should be:

Inspected for cleanliness & damage

Why should bedding not be overfilled?

It can interfere with normal cage function & animal observation

Which document provides standards for Laboratory animal housing?

Guide for the Care & Use of Laboratory Animals

Proper cage density is important because it:

Supports animal welfare & research quality

Which statement best describes an IVC?

Provides individually filtered ventilation

Environmental enrichment should:

Be provided unless restricted by the research protocol

Which animal observation requires prompt reporting?

Labored breathing

Cage sanitation is important because it:

Removes waste & reduces pathogen exposure

A cage with broken plastic should:

Be removed from service

During cage changing, technicians should:

Minimize stress to animals

Which cage system generally requires more specialized maintence?

Individually ventilated cage

The most appropriate action when a cage card does not match the animals is to:

Notify the supervisor & follow SOPs

Which is the best example of maintaining biosecurity during cage changes?

Changing gloves as required by SOP

If an animal appears dehydrated during the cage check, the technician should first:

Ensure the water source is functioning & report the concern

Why are routine cage changes scheduled?

To reduce odors, waste, and ammonia accumulation

Which statement abt social housing is correct?

Compatible social species should generally be grp housed unless there is a scientific or veterinary reason

A technician finds moldy feed in a food hopper. What is the BEST action?

Remove & replace the contaminated feed & report the issue according to SOP

Which practice best protects both the animals & the integrity of the research?

Following SOPs consistently during husbandry procedures

Which statement BEST summarizes proper caging practices?

Animal housing should provide safety, sanitation, appropriate enrichment, & support the animals’ physical & behavioral needs while meeting research requirements

The environment immediately surrounding an animal inside its cage is called the:

Microenvironment

Which environment is directly controlled by the facility’s HVAC system?

Macroenvironment

HVAC stands for:

Heating, ventilation, & air conditioning

The primary purpose of an HVAC system is to:

Maintain environmental conditions

Air changes per hour (ACH) refers to:

The number of times room air is replaced each hour

Most laboratory animal rooms receive approximately:

10-15 ACH

HEPA filters remove at least:

99.97% of particles measuring 0.3 um

Positive-pressure rooms are primarily designed to:

Protect animals from outside contamination

Negative-pressure rooms are commonly used for:

Quarantine or biohazard areas

Which cage system provides filtered air directly to each cage?

Individually ventilated cage (IVC)