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Helicase
It breaks the hydrogen bonds between the base pairs, Separating the two DNA strands, turning the double helix into two single strands so they can be copied. Creating Y-shaped structure called the replication fork where new DNA will be built
Single-Stranded Binding Proteins (SSBPs)
They stick to the single strands and hold them apart, so the hydrogen bonds don’t reform. If the strands came back together, replication couldn’t happen.
Topoisomerase
It moves ahead of helicase and makes small cuts in the sugar-phosphate backbone of the DNA. This relieves tension and prevents supercoiling or tangling as the DNA unwinds.
DNA
Deoxyribonucleic Acid