B1.3.2 - Benedict's Test

What does the Benedict’s Test test for?

reducing and non-reducing sugars

What test tests for reducing and non-reducing sugars?

Benedict’s test

Are monosaccharides reducing or non-reducing sugars?

reducing

Are disaccharides reducing or non-reducing sugars?

either

Is glucose a reducing or non-reducing sugar?

reducing

Is galactose a reducing or non-reducing sugar?

reducing

Is fructose a reducing or non-reducing sugar?

reducing

Is lactose a reducing or non-reducing sugar?

reducing

Is maltose a reducing or non-reducing sugar?

reducing

Is sucrose a reducing or non-reducing sugar?

non-reducing

What is the first step in a Benedict’s test?

place the substance in a boiling tube

What state of matter must the substance be in a Benedict’s test?

liquid

What is the next step in a Benedict’s test after placing the substance in a boiling tube?

add Benedict’s solution

What is the next step in a Benedict’s test after adding Benedict’s solution?

heat it in a water bath

What would a positive result of a Benedict’s test be?

colour change from blue to red

What does a blue result mean in an initial Benedict’s test?

no reducing sugar

What does a green/yellow result mean in an initial Benedict’s test?

traces of reducing sugar

What does a orange result mean in an initial Benedict’s test?

moderate amount of reducing sugar

What does a brick red result mean in an initial Benedict’s test?

lots of reducing sugar

What does an initial Benedict’s test test for?

reducing sugar

What does a secondary Benedict’s test test for?

non-reducing sugar

What is the first step in a secondary Benedict’s test?

boil in dilute hydrochloric acid

What is the next step in a secondary Benedict’s test after boiling in dilute hydrochloric acid?

add sodium hydrogen carbonate

Why is hydrochloric acid used in a secondary Benedict’s test?

to hydrolyse the non-reducing sugar

Why is sodium hydrogen carbonate used in a secondary Benedict’s test?

to neutralise the solution

What is the next step in a secondary Benedict’s test after adding sodium hydrogen carbonate?

repeat the test for reducing sugars

What does a blue result mean in a secondary Benedict’s test?

no non-reducing sugar

What does a green/yellow result mean in a secondary Benedict’s test?

traces of non-reducing sugar

What does an orange result mean in a secondary Benedict’s test?

moderate amounts of non-reducing sugar

What does a brick red result mean in a secondary Benedict’s test?

lots of non-reducing sugar