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B1.3.2 - Benedict's Test
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1
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What does the Benedict’s Test test for?
reducing and non-reducing sugars
2
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What test tests for reducing and non-reducing sugars?
Benedict’s test
3
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Are monosaccharides reducing or non-reducing sugars?
reducing
4
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Are disaccharides reducing or non-reducing sugars?
either
5
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Is glucose a reducing or non-reducing sugar?
reducing
6
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Is galactose a reducing or non-reducing sugar?
reducing
7
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Is fructose a reducing or non-reducing sugar?
reducing
8
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Is lactose a reducing or non-reducing sugar?
reducing
9
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Is maltose a reducing or non-reducing sugar?
reducing
10
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Is sucrose a reducing or non-reducing sugar?
non-reducing
11
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What is the first step in a Benedict’s test?
place the substance in a boiling tube
12
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What state of matter must the substance be in a Benedict’s test?
liquid
13
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What is the next step in a Benedict’s test after placing the substance in a boiling tube?
add Benedict’s solution
14
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What is the next step in a Benedict’s test after adding Benedict’s solution?
heat it in a water bath
15
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What would a positive result of a Benedict’s test be?
colour change from blue to red
16
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What does a blue result mean in an initial Benedict’s test?
no reducing sugar
17
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What does a green/yellow result mean in an initial Benedict’s test?
traces of reducing sugar
18
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What does a orange result mean in an initial Benedict’s test?
moderate amount of reducing sugar
19
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What does a brick red result mean in an initial Benedict’s test?
lots of reducing sugar
20
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What does an initial Benedict’s test test for?
reducing sugar
21
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What does a secondary Benedict’s test test for?
non-reducing sugar
22
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What is the first step in a secondary Benedict’s test?
boil in dilute hydrochloric acid
23
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What is the next step in a secondary Benedict’s test after boiling in dilute hydrochloric acid?
add sodium hydrogen carbonate
24
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Why is hydrochloric acid used in a secondary Benedict’s test?
to hydrolyse the non-reducing sugar
25
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Why is sodium hydrogen carbonate used in a secondary Benedict’s test?
to neutralise the solution
26
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What is the next step in a secondary Benedict’s test after adding sodium hydrogen carbonate?
repeat the test for reducing sugars
27
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What does a blue result mean in a secondary Benedict’s test?
no non-reducing sugar
28
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What does a green/yellow result mean in a secondary Benedict’s test?
traces of non-reducing sugar
29
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What does an orange result mean in a secondary Benedict’s test?
moderate amounts of non-reducing sugar
30
New cards
What does a brick red result mean in a secondary Benedict’s test?
lots of non-reducing sugar
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