Replication enzymes

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7 Terms

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Helicase

An enzyme that unwinds the DNA double helix during replication, separating the two strands to allow access for other replication enzymes.

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Topoisomerase

Relieves overwinding strain ahead of replication forks by rejoining DNA strands

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Single strand binding protein

Binds to and stabilizes single - stranded DNA until it is used as a template

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primase 

Synthesizes an RNA primer at 5’ end of leading strand and 5’ end of each okazaki fragment 

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DNA Poly III

The main enzyme responsible for DNA synthesis during replication, adding nucleotides to the growing DNA strand in a 5’ to 3’ direction.

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DNA Poly I

Removes RNA nucleotides of primer and replaces them with DNA nucleotides, completing the DNA strand synthesis.

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DNA Ligase

Enzyme that joins Okazaki fragments and seals nicks in the DNA backbone during replication.