Sterilization and Disinfection

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Description and Tags

https://www.cdc.gov/dental-infection-control/hcp/summary/sterilization-disinfection.html

Last updated 2:37 AM on 6/20/26
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17 Terms

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Reprocessing

Dental practice should have policies for

  • Containing, transporting, and handling contaminated instruments

  • Each instrument has its own reprocessing instructions

  • Single use devices = no reprocessing instructions and dispose of

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Classification of Patient-Care Items

Patient-care items are classified based on risk of infection transmission:

  1. Critical items

  2. Semicritical items

  3. Noncritical items

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Critical patient care items

Items that penetrate soft tissue or bone.

Examples

  • Surgical instruments

  • Periodontal scalers

Infection Risk

  • Highest risk

Processing

  • Must always be heat sterilized

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Semicritical Items

Items that contact

  • Mucous membranes

  • Non-intact skin (exposed skin, chappeed, dermatitis)

Examples

  • Mouth mirrors

  • Amalgam condensers

  • Reusable impression trays

Infection Risk

  • Lower than critical items

Processing

  • Heat sterilization is preferred (most are heat tolerant)

  • If not heat tolerant — replace w/ heat tolerant alternative or disposable

  • If neither is available → High-level disinfection (minimum).

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Noncritical Items

Items that contact intact skin only.

Examples

  • Radiograph cone/head

  • Blood pressure cuff

  • Facebow

Infection Risk

  • Lowest risk

Processing

  • Cleaning only

If visibly soiled:

  • Clean

  • Then disinfect using an EPA-registered hospital disinfectant

Disposable barriers may also be used.

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Important exception: Dental handpieces

  • Low speed motors

  • Reusable prophylaxis angles

Although classified as semicritical, they:

  • Become contaminated during use.

  • Must always be heat sterilized between patients.

  • Do NOT use high-level disinfection or surface disinfection alone.

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Digital Radiography Sensors

Classified as semicritical.

During use

  • Protect with an FDA cleared barrier

Between patients

  • Clean

  • Heat sterilize or

  • High-level disinfect (if manufacturer allows)

If sterilization/high-level disinfection is not possible:

  • Keep barrier protection

  • Clean and disinfect using an EPA-registered hospital disinfectant with intermediate-level (tuberculocidal) activity

Always follow the manufacturer's reprocessing instructions

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Instrument Processing

Cleaning

  • Clean before sterilization or disinfection

Why?

Blood, saliva, and debris can hinder effectiveness of sterilization or disinfection

Automatic cleaning equipment

  • Ultrasonic cleaner or washer disinfector — used to remove debris or improve cleaning

After cleaning

  • Inspect instrument, wrap into packages, heat sterilized

  • Label each package (sterilizer used, cycle, date, expiration)

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Sterilization monitoring

  1. Biological monitoring

  2. Mechanical monitoring

  3. Chemical monitoring

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Biological monitoring

Most reliable method — kills high resistant bacterial spores (bacillus species)

  • Must be done at least 1x a week

  • Results are not immediate

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Mechanical monitoring

  • Monitors procedural errors and equipment malfunctions

  • Monitors pressure, temperature, exposure time (gauges, computer displays, prints)

They help record during sterilization records

  • usually the first sign of equipment malfunction

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Chemical monitoring

Uses chemicals that change color when exposed to high temperatures (sterilized conditions)

  • Indicator tape, stripes, package markings

  • Provides immediate result after cycle

  • Must be used inside every package to very sterilizing agent has penetrated package (internal chemical indicator)

  • If cant use then must use external indicator

  • No color change = don’t use instruments

  • Distinguishes processed from unprocessed packages.

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Internal chemical indicators

Single parameter

  • Measures 1 sterilization parameter through time or temperature

Multiparameter

  • Measures 2+ parameters through time+ temp or time + temp + steam

  • More reliable

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Sterilization monitoring

Sterilization must be monitored

  • If problem (uncahnged chemical indicator or positive spore test) — document

  • Sterile supplies must be stored in closed cabinets or wrapped

  • Pacakaging can’t be torn— compromised packages needs to be reprocessed

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Recommendations for sterilization of care devices for dental

  • clean and reprocess equipment before and after use according to instructions

    • If no instructions = might not be multiuse

  • Assign reprocessing responsibilities to DHCP

  • Wear PPE during reprocessing

  • Use mechanical, chemical, and biological monitors

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Environmental infection prevention and control

Important policies

  • Clean before disinfect or sterilization

    • Cleaning: Removes dirt, debris, microorganisms

    • Disinfection: Kills most disease-causing microorganisms but does not necessarily destroy bacterial spores (unlike sterilization).

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Clinical contact surfaces

Frequently touched urface — can be contaminated during treatment

  • Light handles, bracket trays, unit switches, computer keyboards/ equipment, dental chair controls

  • Can transfer to DHCP, patients, hands etc

Prevention

  • Disposable surface barriers between patients

  • No barriers? —> clean surface + disinfect w/ EPA registered hospital disinfectant

  • Has blood? —> Use intermediate level disinfectant (tuberculocidal claim)

Floors, walls, sinks = low infection risk

  • Use soap and water + disinfect if blood stain