Studied by 8 people
What is the TCA for?
Produces energy from glucose products
Net reaction of TCA?
2 Acetyl-CoA + 6 NAD+ + 2 FAD + 2 ADP → 4CO2 + 6 NADH + 2 FADH2 + 2 ATP + 6 H+
Where does the TCA occur?
Mitochondrial matrix
What are the 3 committing steps of TCA? Name the enzymes.
Citrate Synthase, Isocitrate dehydrogenase, and a-keotglutarate dehydrogenase
What regulates citrate synthase?
Positive: Energy charge, need for TCA products
Negative: ATP, NADH, Citrate, Succinyl-CoA
What regulates Isocitrate Dehydrogenase?
Positive: Energy Charge, need for TCA Products
Negative: ATP, NADH
What regulates a-ketoglutarate dehydrogenase?
Positive: Need for TCA products
Negative: NADH, Succinyl-CoA
What is the chemical logic of the TCA?
Citric acid intermediates are more easily oxidized than acetate (product of pyruvate) and therefore makes e- transfer more efficient
What is the pattern for Carbon tracing from Glucose?
Carbon 3/4: 100% Lost before TCA
Carbon 2/5: 100% Lost in round 2 in TCA as CO2
Carbon 1/6: 50% Lost in round 3, 25% in round 4, …
What in an anaplerotic reaction?
A reaction that replenishes TCA intermediates
Why is the TCA amphibolic?
Does both anaplerosis and cataplerosis to use and regenerate intermediates - cyclical
How is Oxaloacetate replenished?
PEP Carboxylase and Pyruvate Carboxylase
What other process is Pyruvate carboxylase used in?
Gluconeogenesis
What activates pyruvate carboxylase?
Acetyl-CoA
What cofactor does pyruvate carboxylase use?
Biotin
