BIOC2302 Cell growth with EGF

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6 Terms

1
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what is DMSO

solvent used in cell cultures, dissolves a range of compounds and protects cells from damage during freezing and thawing (cryoprotectant)

2
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what was used to image cells after giving EGF with and without inhibitor (and control with neither)

EVOS microscope

3
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what is phase contrast microscopy

technique that enhances contrast of transparent and colourless specimens

making them more visible under a microscope (dont need stain samples)

4
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what 4 different filters are images taken with in the EVOS microscope

phase contrast, GFP, RFP and all 3 combined (overlay)

5
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what is Image J/FIJI

cell counting software (count red, yellow and green and non-fluorescent cells)

6
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