Bacterial Conjugation

conjugation

purposeful transfer of genetic material from one bacteria to another via pilus

transformation

partial genome uptake rom naked DNA

transduction

transfer via viral genome

is genetic material transfer in bacteria reciprocal?

no, one cell is the donor and the other is a recipient

fertility factor

A bacterial episome whose presence determines donor ability (maleness).

unidirectional inheritance

rolling circle replication

a circular double stranded DNA replicates to produce linear DNA, unidirectional

copy of F remains in the donor and another appears in recipient

plasmid

circular dna not part of chromosome or genome

higher

f factor has ___________ freq of replication than the genome

Hfr strains

high frequency of recombination strains

Hfr have the f factor integrated into the chromosome

what makes an Hfr strain different than a regular F factor?

episome

when a plasmid integrates into chromosomes

loci of insertion

what determines the freq of recombination of an Hfr?

F+ x F-

recipient becomes F+ but low recombination with other genes

Hfr x F-

recipients stays F- but HIGH recombination with other genes

crossovers integrate parts of the transferred donor fragments into the genome

what is the roll of recombination of donor mating in bacteria?

exconjugants

cell bearing a donor allele that was taken during recombination

last

where is the part of F that confers donor ability located on in the order of transmission into the recipient?

mapping by using time of entry

what is a method of broad-scale chromosome mapping?

exogenote

incomplete genome from the donor (the F factor or Hfr chromosome)

endogenote

the complete endogenous recipient chromosome

merozygote

a cell which is a partial diploid, containing both an endogenote and an exogenote

two ways methods of DNA transfer during conjugation

chromsome transfer (F-a+ and F-a-)

plasmid transfer (F+a-)

result of recombination during conjugation

doubl-crossover-like event gives rise to reciprocal recombinants of which only one survives

F' plasmid

f plasmid carrying bacterial genomic DNA

Shigella

bacteria that causes bacterial dysentery

R Plasmids

A bacterial plasmid carrying genes that confer resistance to certain antibiotics.

transposon

A transposable genetic element that moves within a genome by means of a DNA intermediate.