Quizbowl Lab Procedures I

protein purification

Chromatography

Stationary phase separates components of mobile phase

Chromatography

Paper version

Chromatography

High pressure liquid version

Chromatography

Adsorbent alumina or silica used

Chromatography

cation/anion exchange resins

Chromatography

Imidazole buffer and nickel-NTA beads

Chromatography

Partition coefficient

Chromatography

Ion-exchange version

Chromatography

Purnell equation

Chromatography

Van Deemter plots

Chromatography

Theoretical plate number

Chromatography

azeotropes

Distillation

“Vacuum” type

Distillation

Perkin triangle

Distillation

Fenske equation

Distillation

Kugelrohr apparatus

Distillation

“Piq” adaptor

Distillation

Measures light absorbed by color sample

Spectrophotometry

cuvettes

Spectrophotometry

Beer’s Law

Spectrophotometry

concentration, path length, and the extinction coefficient

Spectrophotometry

Bausch and Lomb device called Spec 20

Spectrophotometry

measures transitions between electronic energy levels

Spectrophotometry

Determines isosbestic point

Spectrophotometry

Woodward-Feiser rules

Spectrophotometry

Photosystems named after output of this

Spectrophotometry

Start at 214 or 253 and add multiples of 5 or 30

Spectrophotometry

Complexometric form

Titration

HETP

Chromatography

Eddy diffusion

Chromatography

UCM hump

Chromatography

2-D form

Chromatography

“Destructive” form

Distillation

Several noble gases discovered via

Distillation

“Back” form

Titration

Eriochrome Black T

Titration

Winkler Test

Titration

“Reverse” form uses excess silver nitrate

Titration

Craig tubes

Filtration

Celite

Filtration

Pasteur pipettes

Filtration

final step of a recrystallization

Filtration

Buchner funnels

Filtration

“Vacuum” type** not distillation

Filtration

Odd atomic masses

NMR

Used in MRI’s

NMR

heavy water acts as solvent

NMR

Dewar-suspended superconductor

NMR

n-plus-1 rule

NMR

tree diagrams

NMR

Paul Lauterbur

NMR

RF current

Mass Spectroscopy

Odd number of Nitrogen atoms

Mass Spectrometry

Electrospray preparation

Mass Spectrometry

“Aerosol” form

Mass Spectrometry

“Accelerator” form

Mass Spectrometry

Identifies free swim state of trematodes

Mass Spectrometry

Penning trap

Mass Spectrometry

Fourier transform

Mass Spectrometry

Sinapinic acid

Mass Spectrometry

“Tandem” form

Mass Spectrometry

“Time of flight” form

Mass Spectrometry

MALDI procedure

Mass Spectrometry

“muon-catalyzed” form

Fusion

palladium electrode

Fusion

“Inertial confinement” form

Fusion

Lawson criterion

Fusion

Troyon limits

Fusion

ITER

Fusion

Efficiency found via “triple product”

Fusion

“Acoustic” type

Microscopy

Microcantilever

Microscopy

liquid ethane

Microscopy

“Cryogenic” type

Microscopy