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What happens to tubes without additives before centrifugation?
They allow the blood to clot naturally
Why must tubes without additives be centrifuged?
To separate blood components after clotting
What is aliquoting?
Removing the liquid portion (plasma or serum) after centrifugation and transferring it into a separate tube for testing
Why is following the order of draw important?
It maintains specimen integrity and reduces the risk of sample contamination or rejection
What is chain of custody?
A strict protocol for tracking forensic specimens from collections to testing
What types of tests require chain of custody?
Blood alcohol testing, drug testing, forensic poisoning, and paternity DNA testing
What tests require prewarmed tubes?
Cold agglutinin and cryoglobulin
What does without stasis mean?
Specimen must be collected without a tourniquet
Which test must be collected without stasis?
Lactic acid
Why must some specimens be protected from light?
Light exposure can degrade certain substances
Which tests are light-sensitive?
Bilirubin, vitamin B6, vitamin K, vitamin B12
How are light-sensitive specimens protected?
Places in a tin foil mold or an amber-colored bag
Why must some specimens be kept warm after collection?
To maintain specimen integrity and prevent precipitation or degradation
Which tests require warmed specimens?
Cold agglutinin, cryofibrinogen, cryoglobulin
Why must some specimens be chilled immediately?
To prevent cellular separation and slow biochemical reactions that could alter results
Which test requires chilling?
Ammonia
What does frozen specimen require?
The sample must be frozen immediately after the blood draw
Which tests require frozen specimens?
ACTH, calcitonin, factor 9, gastrin, and hepatitis C
What are timed specimens?
Specimens collected at a specific time of day to monitor conditions or treatment effectiveness
What are peak levels?
The highest concentration of medication in the blood
What are trough levels?
The lowest concentration of medication in the blood