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Why Kinetics?
Structure - Function - Rates
Rates are affected when change amount and componenets
Michaelis - Menten equation
Hyperbolic:
Vo (Y-axis) = Rate enzyme catalyzes
S (X-axis) = The concentration of enzyme
Vmax = all enzyme it has is saturated and for it to go any higher there needs to be more enzymes added on
Km is inverse to affinity
High Km = Low affinity
Low Km = High affinity
Km = ?
[S] at ½ Vmax
y intercept = ?
1/vmax
x - intercept = ?
-1 / Km
Turnover number
= the number of substrates converted to product per sec
Kcat = Vmax / Et
Catalytic Efficiency
Kcat / Km
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