culture media

Amie's Media

Transport media, to maintain viability of the organisms from the source to the laboratory

Acetamide Agar

Differentiation of nonfermentative gram negative bacteria – Pseudomonas aeruginosa

Alkaline Peptone water

Enrichment media for Vibrio

Bacteroides Bile Esculiun (BBE)

Selective and differential media. Selective for Bacteroides. Used to differentiate bile resistant from bile sensitive Bacteroides

Bile Esculin Agar

Nutrient agar base media with ferric citrate. Differential isolation & presumptive identification of Group D Streptococci and Enterococci

Bird Seed Agar

Selective and differential medium used in the identification of Cryptococcus neoformans

Brain Heart Infusion Broth (BHI)

High protein media for growing a wide variety of bacteria

Bismuth Sulfite Agar

Used for isolation of Salmonella typhi. Produces black colonies. Not recommended for isolation of all Salmonella

Blood Agar Base (BA)

Cultivation of fastidious microorganisms, used in determining haemolytic reactions

Blood Cystine Dextrose Agar

For growing Francisella tularensis

Bordet-Gengou Media

Isolation of Bordetella pertussis

Brilliant Green Agar (BGA)

Selective media for isolation of Salmonella other than S. typhi. Not recommended for Salmonella

Brilliant Green Bile Agar

Isolation, differentiation and enumerating coliform bacteria

Buffered Charcoal Yeast Extract Agar

Enrichment for Legionella spp. Supports the growth of Francisella and Nocard9ia

CLED Agar (Cystine Lactose-Electrolyte-Deficient)

Presumptive identification of microorganisms from urine

Chapman Stone agar

Selective media for the isolation of Staphylococcus

Campy-Blood Agar

Selective for Campylobacter spp.

Campylobacter Thioglycollate Broth

Selective holding medium for recovery of Campylobacter spp.

Cary and Blair Media

Transport media

Cetrimide Agar Base

Selective isolation and identification of Pseudomonas aeruginosa

Cefoperazone, Vancomycin, Amphotericin (CVA)

Selective media for isolation of Campylobacter spp.

Cefsulodin-Irgasan-Novobiocin (CIN)

Selective for Yersinia spp. Maybe useful for isolation of Aeromonas spp.

Chocolate Agar (CAP)

Cultivation of Haemophilus spp. and pathogenic Neisseria

Chopped Meat Glucose

Used for primary isolation of anaerobes. Used for Clostridium spp.

Columbia Colistin Nalidixic Acid Agar (CNA)

Selective isolation of gram positive cocci

Columbia Agar Base

Highly nutritious, general purpose medium for isolation of fastidious and non fastidious microorganisms

Corn Meal Agar with 1% Tween 80

Used to stimulate production of Chlamydospores of Candida albicans

Cystine Tellurite Blood Agar

Isolation of Corynebacterium diphtheria

Cystine Trypticase Agar (CTA)

CTA with carbohydrates added is excellent for determining the fermentation reactions of groups such as Neisseria

Chocolate Agar

Cultivation of Haemophilus spp. and pathogenic Neisseria spp.

Chromogenic Agar

Designed to optimize growth and differentiation a specific type of organism. Routinely used to identify yeast, MRSA, and other organisms

Cycloserine cefoxitin Fructose agar (CCFA)

Selective media for Clostridium difficile

Desoxycholate Agar

Differential medium for gram negative enteric bacilli

Deoxycholate Citrate Agar

For isolation of Salmonella and Shigella

Dieudonnes media

Selective media for Vibrios

“E” agar and Edward Hayflick agar

Selecxtive media for the isolation of Mycoplasma and Ureaplasma

Egg Yolk Agar

Used for the isolation and identification of Clostridium

Eosin Methylene Blue (EMB)

Isolation & differentiation of lactose fermenting & non-lactose fermenting enteric bacilli

Ellinghausen, McCullough Johnson and Harris Media (EMJH)

Selective media for Leptospira Interrogans

Fletcher’s Medium

Enriched, semisolid medium used for Leptospira

Fetal Bovine serum with Vancomycin

Selective media for H. ducreyi

Fletcher’s medium

For isolation of Leptospira

Feeley Gorman Medium

Legionella spp.

Fildes Enrichment

Used for isolation of H. influenzae

GC Medium Base

With various additives in isolating and cultivating Neisseria gonorrhoeae

Gram Negative Broth (GN)

Selective enrichment (liquid) medium for enteric pathogens - Salmonella and Shigella

Granada Medium

Streptococcus agalactiae

Hektoen-Enteric (HEA)

Differential, selective media for the isolation and differentiation of Salmonella and Shigella spp. from other enteric bacilli

Human Blood Bilayer tween

Selective media for the isolation of Gardnerella vaginalis

Kelly’s Medium

Borrelia spp.

Lactose Broth

Detection for the presence of coliform organisms

Leoffler’s Coagulated Serum

Used for C. Diphtheria

LIM Broth

Selective enrichment of group B streptococci esp. from genital specimens

Lysine Iron Agar

Biochemical test used to differentiate enteric organisms

Lowenstein Jensen Media

Used for the isolation of Mycobacterium tuberculosis and other Mycobacterium. Malachite green is used to inhibit all organisms except Mycobacterium

Mac Conkey Agar

Peptone base media with lactose. Used for the isolation and differentiation of lactose and non-lactose fermenting enteric bacilli

Mac Conkey Sorbitol Agar

A modified Mac Conkey that contains D-sorbitol instead of lactose as primary carbohydrate. For the selection and differentiation of E. coli O157:H7 in stool specimens

Mannitol Salt Agar

Selective isolation of Staphylococci

Malonate Broth

Used to differentiate Enterobacter from Escherichia

Martin Lewis Agar

Isolation of Neisseria from specimens containing mixed flora

Mc Bride Media

For the isolation of Listeria monocytogenes

Middlebrook 7H10 or 7H11

For the isolation of Mycobacterium tuberculosis

Modified Kelly’s medium or Barbour Stoenner Kelly

Selective media for the isolation of Borrelia species

MRVP broth

Used in Methyl Red and Vogues Proskauer tests. These are biochemical tests that help in the identification of enteric bacilli

Mueller Hinton Agar

For antimicrobial susceptibility testing

New York City Agar

Selective of Neisseria gonorrhea

Nutrient Agar

General media for the enumeration of organisms in water, sewage, feces and other materials

OF Basal Medium

Determination of oxidative and fermentative metabolism of carbohydrates by gram negative bacteria

Petragnani’s Media

Same use as Lowenstein Jensen with same ingredients except that this contains more malachite green

Phenylethyl Alcohol Agar (PEA)

Selective isolation of gram positive cocci and anaerobic gram negative bacilli

Potato Dextrose Agar

Cultivation and enumeration of yeasts and molds

Rappaport-Vassiliadis Medium

Detection of Salmonella in feces and food products

Regan Lowe/Charcoal Cephalexin Agar

Enrichment and selective media for Bordetella pertussis

Sabouraud Dextrose Agar

Used for fungus culture

Salmonella-Shigella Agar (SSA)

Selective for Salmonella and Shigella

Schaedler Agar

Non-selective media for the recovery of anaerobes and aerobes

Shepard's Medium (SP4), A7B, E-agar

Mycoplasma

Selenite Broth

Enrichment for the isolation of Salmonella spp.

SIM Medium

Biochemical test used to differentiate enteric bacilli

Simmon Citrate Agar

Biochemical test used for the differentiation of gram negative bacteria

Skirrow Agar

Selective for Campylobacter spp.

SPS Agar (Sulfite Polymyxin Sulfadiazine)

Detection and enumeration of Clostridium perfringens in food

Streptococcal Selective Agar

Selective for Streptococcus agalactiae in female genital specimens

Tetrathionate Broth Base

Selective for Salmonella from feces, urine, foods and other materials of sanitary importance

Thayer Martin Agar

Selective for Neisseria gonorrhoea and Neisseria meningitidis

Thioglycollate Broth

Supports growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms

Thiosulfate Citrate Bile Salts (TCBS)

Selective and differential for Vibrios

Todd Hewitt Broth supplemented with Antibiotics (LIM)

Selective and enrichment for Streptococcus agalactiae in female genital specimens

Tomato Juice Agar

Lactobacillus acidophilus

Tryptic Soy Agar

Isolation and cultivation of nonfastidious and fastidious organisms

Trypticase Soy Broth (TSB)

Enrichment broth used for subculturing various bacteria from primary agar plates

Tap Water Agar (Bacto Agar)

To differentiate Actinomycetes (Nocardia, Rhodococcus, Gordonia, Tsukamurella, Streptomyces, Actinomadura, Dermatophilus, Nocardiopsis)

Triple Sugar Iron Agar (TSI)

Media used in biochemical tests to differentiate gram negative bacilli especially enteric bacilli

Xylose Lysine Desoxycholate Agar (XLD)

Isolation and differentiation of Salmonella and Shigella spp. from other enteric bacilli

Urea Agar Base

Biochemical test which is ideal for identification of Proteus

V Agar

Isolation and differentiation of Gardnerella vaginalis

Gentamicin BAP (GBAP)

Streptococcus pneumoniae

Bacitracin CAP (BCAP)

Haemophilus influenzae

Cystine Trypticase agar

Neisseria (for confirmatory)

McCoy cells

Chlamydia trachomatis