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Ecology
the study of the interconnections and interrelationships between organisms and their environment
Ecology can study relationships between the ___________ and ____________ species
same; different
Abiotic
non-living
Levels of organization
the different levels at which biology is studied
The levels of organization are…
atoms, cells, organisms, population, community, ecosystem, biosphere
Nested hierarchy
when the larger part of a system are made up of the smaller parts, levels of organization is an example of this
Sample/ population size (N)
the size of the sample or population being studied
Lambda (λ)
population growth rate, the ratio of population sizes in a closed population, (Nt+1/Nt)
Population dynamics
changed to a population over time, works in a cycle, growth decline stability and recovery
Carrying capacity (k)
the largest the population can stably be
Population size and dynamics are controlled by _________ factors, ________ _________ factors, and ____________
biotic; abiotic environmental; dispersal
BD model
the birth and death model, only for a closed population, the current population- those who died + those born
BIDE model
birth immigration death emigration, current population + birth +immigration - death - emigration
Birth rate (b)
the number of people born / population
Death rate (d)
the number of deaths / population
Population dynamics are determined by _______ _ ________
birth v. death
Community
a group of multiple species living together at the same place and time
Geographic range
an area made up of multiple communities
Intraspecific interactions
interactions between members of the same species
Interspecific interactions
interactions between members of different species
Predation
common negative direct interaction in a community, hunting
Competition
common negative direct interaction in a community, fighting for the same food
Indirect interactions
effecting another species through forces where they do not interact
Ecosystems
communities with physical, chemical , abiotic interactions, and physical factors
Physical features
land and geography, studied by geologists geographers and climatologists
Anthropogenic ecosystems
ecosystems where humans are major influences of physical and abiotic features
Anthropogenic ecosystems
ecosystems where humans are major influences on physical and abiotic features
Agroecosystems
ecosystems where human agriculture occurs
Succession
the series of changes in ecosystem conditions that occur after a major abiotic or biotic event or change, like a disturbance
Disturbances
major changes to an ecosystem that stress species, typically much of the biomass is changed or killed, typically kill much of a communities foundation species
Foundation species
species that play a major role in a community by providing habitat, food, etc. for many species
Human disturbance
a large change done by humans to a community
Clearcut
a human disturbance, all trees cut down and usually left to rot
Slash and burn
a human disturbance, a farming technique where an area is cut down and burned to release nutrients into the soil
Selective logging
a human disturbance, removal of just the valuable trees, leaves other trees intact
Primary succession
succession from a completely new substrate, starts with no biomass, ex. after a large volcano, fire, or glacier retreat
Nitrogen fixing plants
usually the first plants of a primary succession, use nitrogen to create their own fertilizer
Secondary succession
disturbances leaving residual biomass below or above ground
Pulse disturbance
a pulse of damage, death, or loss of biomass to a community
Press disturbance
a disturbance that does not allow succession to take place, ex. continuously grazing a field
Habitat mosaics
constant press disturbance that only allows some areas to undergo succession, creates a fragmented looks
habitat fragmentation
little pieces of habitat left behind by human press disturbances, leaves gaps between small fragments
Habitat edges
the outside line of fragmented habitats, causes edge effects
Soil aeration
the process of increasing air and water flow in the ground
Ecosystem engineer
things with pervasive qualities, alter the physical environment, ex. humans and elephants
Savanna
a grassland ecosystem with trees and shrubs
Herbivore outbreak
a biotic disturbance, many herbivores, eat and kill many trees and plants
Sequencing the human genome found the ___________ ___ _________ ________
sequence of human genes
“Omics”
fields related to the biggest biology and medicine topics, focus on a complete set of a biologicas molecule
Genomics
the field of biology that focuses on studying all of the DNA or the genome of an organism
Genomicists
people who study genomes
Genomics studies the number of _______, number of _______, and what they do
chromosomes; genes
the nuclear genome is __ % ____- _______ coding
97; non-protein
the mitochondrial genome is made up of ___ _________ chromosome, __ genes, which __ % are non-coding
one circular; 37; 3
Transcriptome
all of the RNA transcripts in a cell or which can be produced by a genome
Humans have _________ RNA transcripts
120,000
Regulatory RNA
binds to mRNA to change its function, changes transcription and ultimately protein production
Proteome
the complete set of proteins in a cell
Proteomics
the science and technology used to study proteomes
Metabolics
the study of small things created through metabolism
Microbiome
a community of microorganisms that live in a shared space
Metagenomics
the focus of genes that are very different between species, used to study microbiomes
Bioinformatics is also known as ____________ _________
computational biology
Bioinformatics
analyzes and stores DNA sequences
Annotations
additional notes and information about a DNA sequence
Spirochete
spring like shape
Sequencing begins with many ________ of a ________
copies; genome
The _______ step of DNA sequencing is _________ ________ each __________ into smaller pieces
first; randomly chopping; chromosome
Each DNA fragment in DNA sequencing is about around ____ base pairs
100
The _______ step in DNA sequencing is _______ DNA fragments into __________ _________ DNA
second; denaturing; single stranded
_________ is used to denature DNA in DNA sequencing
Heat
The ______ step in DNA sequencing is _______ an ________ ______ to the end of the DNA strands
third; ligating; adapter sequence
Adapter sequence
used in DNA sequencing, added to single stranded DNA, acts as a target sequence for a complementary primer
The ________ step in DNA replication is using a ______ like reaction to make _________ of the ______ ________
fourth; PCR; copies; DNA strands
The _________ step in DNA sequencing is ___________ or finding the ________ in each ________ _________
fifth; sequencing; bases; DNA fragment
The _______ step in DNA sequencing is _________ __________ or _________ the fragments into their ___________ sequence
sixth; genome assembly; organizing; orginal
Alignments
the original sequences made from DNA sequencing
Contiguous stretches (contigs)
a set of DNA fragments that overlap
De novo sequencing
the sequencing of a genome for the first time
Diploids are more complicated to sequence because of ___________ chromosomes, _________ genomes, and can be heterozygous or have __________ in their sequence
multiple; larger; differences
Reference genome
the first sequenced genome of an organism, acts as an example set of genes in a species, not fully representative, can have errors, regularly changed and updated
TATA box
similar to a -10 -35 sequence, helps the promoter bind to the DNA, upstream of the coding sequence, binds general transcription factors
General transcription factors
transcription factors involved in RNA pol binding to genes, on almost every gene, turn gene transcription on and off
Specific transcription factors
transcription factors involved in RNA pol binding to specific genes, turns gene transcription up or down
TFIID
a general transcription factor that binds to all promoters, binds directly to the TATA box, binds before RNA pol
Transcription initiation complex
TFIID binds to the TATA, additional factors bind, RNA pol binds, additional factors bind
The order of the initiation complex is:
regulatory protein binding site, general protein binding site, RNA pol binding site, and transcribed sequence
Enhancers and Silencers
short sequences where specific transcription factors bind on double-stranded DNA either enhancing or limiting DNA transcription, can be near or far from the promoter, can be located in an intron for another gene
TATAless genes
eukaryotic genes without a TATA box
DNA bending
DNA bends so the enhancer or silencer is across from the promoter
A mutation in an intron can affect a protein if it affects an _________ or _______
enhancer; silencer
Proteins bind to DNA or RNA through ___________ bonds in ___________ and __________ __________
hydrogen; major; minor grooves
Mononucleotides
A, T, C, G
Di-nucleotides
CC, GG, CG, GC
There are ____ to ____ thousand genes in the human genome
20; 22
The current human reference genome comes from __ people
11
____% of the human reference genome comes from one person, probably of _______ decent
70; African
Pseudogene
dead protein, a segment of DNA that structurally resembles a gene but is not capable coding for a protein, usually from a very mutated gene, can get missed when a genome is being annotated
_____________ genes vary the most between populations
Immune
Methylation
the addition of a methyl group - CH3, covalent but reversible