sample preparation 2.1 and microscopy

what two lenses does a light microscope have?

an eyepeice lens and an objective lens

what image does the objective lens produces?

a magnified image

what are the 4 different ways which samples can be prepared?

dry mount, wet mount, squash slide, smear slide

how are samples prepared in a dry mount?

solid specimens are sectioned thinly, they are then placed in the centre of the slide and a cover slip is placed over the sample

examples of dry mounts?

hair, pollen, parts of insects, petals

how are samples prepared in a wet mount?

specimens are suspended in a liquid, a cover slip is then placed at an angle

what is a wet mount used for?

aquatic samples and other living organisms

how is a sample prepared in a squash slide?

a wet mount is firstly prepared, then the cover slip is gentley pressed down over the sample.

why does care need to be taken in squash slides?

so the cover slip does not break

example of squash slides?

root tips to look at cell division

how is a sample prepared in a smear slide?

the edge of a slide is used to smear the sample which creates a thin even coating another slip is then placed over the sample

what is an example of a smear slide?

sampling blood to view blood cells