Bio 180 - (Prokaryotes, Protists, and Fungi)

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Biology

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1

How are prokaryotes good for you?

  • Hygiene Hypothesis: proposes that the stimulation of the immune system by microbes protects from the development of inflammatory diseases.

  • Food

  • Mutualistic/ Commensals relationships ex.) bacteria living on the roots or leaves of a plant get nutrients from the plant and, in return, produce substances that protect the plant from pathogens.

  • Nutrient cycling: Recycling CO2

  • Electricity: harness energy from microbes

  • Bioremediation: aids in cleaning up environment.

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What are endotoxins?

a toxin that is present inside a bacterial cell and is released when the cell disintegrates. (BREAKS APART, then RELEASES TOXIN)

ex.) Salmonella typhi

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What are exotoxins?

Secreted by bacteria. (Just RELEASES TOXIN and doesn’t BREAK APART)

ex.) Vibrio cholera, H. Pylori, Streptococcus B

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4

Who is Robert Koch?

Conducted studies on illnesses caused by microorganisms. Found out that microorganisms may be a leading cause of an illnesses within a person. Confirmed the Germ Theory.

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What are the 3 domains of prokaryotes?

Bacteria, Archaea, and Eukarya.

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General features of prokaryotes?

  • basic and small

  • no nucleus

  • no membrane bound organelles

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Microbiome

Human microbiome (found on/ within humans). If you possess an unhealth microbe on your body, you will become more susceptible to things within the environment. Ex.) asthma

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Who is Alexander Fleming?

Creator of penicillin and revolutionized medicine with his discovery of using “bacteria” within medicine.

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9

Common shapes found in bacteria

  • Coccus: circle

  • bacillus: Rod

  • spirillum: Spiral

  • vibrio: curved rod (check mark)

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External autonomy on prokaryotes

  • Capsule: stick, defend, Biofilm

  • Pili: Conjunction

  • Fimbriae: Stick, numerous

  • Flagella: helps in movement, motor, injects toxins

  • Cell Wall Plasma membrane

  • Gram Staining: (+): Purple and think and (-): Pink and thin

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Internal autonomy in prokaryotes

  • Nucleoid: main genetic material found in this region

  • Plasmids: additional genetic material

  • Cytoplasm ribosomes: build cytoplasm proteins

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Metabolism in prokaryotes

  • Photoautotroph: cells that capture light energy, and use carbon dioxide as their carbon source.

  • Chemoautotroph: sulfur-oxidizing bacteria, nitrogen-fixing bacteria and iron-oxidizing bacteria, found in oceans.

  • Photoheterotroph: organisms that capture light energy to convert to chemical energy in the cells

  • Chemoheterotroph: down organic wastes and the remains of dead organisms. They play vital roles as decomposers and help recycle carbon and nitrogen.

  • Aerobic v. anaerobic: (Aero)—> with O2, (anae): without O2

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Binary Fission

copying the chromosome and separating one cell into two; making a copy of oneself.

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Mutation

Change in genetic code.

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Transformation

the prokaryote takes in DNA found in its environment that is shed by other prokaryotes; bacteria get genetic material from environment.

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Transduction (virus)

the process by which a virus transfers genetic material from one bacterium to another.

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Conjugation (sex pili)

bacteria share the same genetic material as other bacteria.

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Horizontal Gene Transfer

the movement of genetic information across normal mating barriers, between more or less distantly related organisms; getting genes from other areas (not just parent cells)

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Extremophiles

an organism/bacterium that is able to live in extreme environments.

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Halophile/Halobacterium

an organism, especially a microorganism, that grows in or can tolerate saline conditions. Lives in high salt concentrations.

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How are prokaryotes different from Eukaryotes?

  • No Nucleus

  • No membrane bound organelles.

  • Different cytoskeleton

  • Circular DNA

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Miasma Theory

The old and inaccurate belief that the air held sickness ; you could get sick by inhaling “bad air”

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Germ Theory

Microbes can in fact cause sickness and disease - and can spread from person to person. Specific microbe for each illness.

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What is an endospore?

An endospore is a dormant, tough, and non-reproductive structure produced by some bacteria. It maintains genetic information and half metabolism until it’s in a proper environment, then it releases spores.

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Biofilm

sticky most outer layer built within proteins. Used for defense and helps in moisture.

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Fimbriae

long filamentous hair-like protein structures located at the surface of bacterial cells. Help attach to other bacteria.

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Flagella

a slender threadlike structure, especially a microscopic appendage that enables many bacteria to swim and move. Microtubes are used within the flagella. The flagella has 3 parts.

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3 parts of the flagella

  • Motor

  • Hook

  • Tail

<ul><li><p>Motor </p></li><li><p>Hook </p></li><li><p>Tail </p></li></ul>
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29

who is Hans Christian Gram?

discovers staining bacteria. Depending on how thick or thin the layer is, the cell will be dyed purple (+, thick) or pink (-, thin).

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Endosymbiotic Theory

Lynn marriese founded the theory. The theory suggests that ancient bacteria evolved into the bacteria of today.

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Reasons backing the Endosymbiotic Theory

  • Ancient DNA in mitochondria and chloroplasts are similar.

  • The membrane is similar to the ancient protist.

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What is a protist?

The first eukaryotes, they are very diverse.

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Reproduction in Protists

Asexual reproduction and binary fission

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Relationships w/other organisms

  • (mutualistic) coral need dinoflagellate to survive (Primary Producers) in photosynthesis carbon sinks and protist absorb carbon.

  • (Algae blooms) kill fish.

  • (Disease) Protists may cause disease.

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Excavata

modified mitochondria, feeding groove, cytoskeleton

  • Diplomonads: Giardia (causes stomach pain)

  • Parabasalids: Trichorrias (causes vaginalis)

  • Euglenozoa: Trypanosoma (african sleeping disease)

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SAR- Stramenopiles

straw like structures

  • Diatoms: sillica

  • Golden Algae

  • Brown Algae: Blade, stipe, holdfast

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SAR - Alveolates

tiny sac under membrane

  • Ciliophora: Paramecium

  • Dinoflagellates: Zoomanthellae

  • Apicomplexans: Malaria

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SAR - Rhizarians

hard outershells photosynthetic and moves with amoeboid projection.

  • Radiolarians

  • Forams/Formaniferans

  • Cercozoans

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Archaeplastida

  • Red algae

  • green algae

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40

How old are fungi?

1.3 trillion

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Hyphae

Underground roots of network. 2 types of hyphae :septate hyphae and coenocytic hyphae.

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septate hyphae

have walls between cells that allow for the passing of nutrients.

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coenocytic hyphae

no walls or membrane between cells

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Mycelium

mass network of hyphae

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Cells walls in fungi

made of chitin

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Mycorhizzae

symbiotic relationship with the roots of plants. The fungi share water with the plants and the fungi in return get the products from photosynthesis.

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