Lab Homework #15- Cytology

What is cytology the study of?

Cytology is the study of cells including their structure, function, how they relate to their environment, and their relationship to one another

When looking at cytological samples such as ear cytology and vaginal cytology where does the condenser need to be positioned?

Raised all the way up

Explain why a slide marker is the best utensil to label slides and why a Sharpie marker should not be used to label slides?

A slide marker is the best utensil to label slides with because they are solvent resistant meaning that it will not smear and will not go into the sample preventing any making of an artifact. A sharpie should not be used because unlike the slide marker it will smear into the sample therefore making an artifact on the slide.

Histology is the study of the microscopic structure of tissues. 

true

Do we heat fix cytological slides? Justify your answer to receive full credit.

We do not heat fix cytological slides because if we do, the heat will burn/destroy the cells leading to a false diagnosis

You obtain ear swabs on Twinkie Jefferson a 4-year-old MN Pomeranian. You've prepared and stained the slide. What information should be used to label the slide? 

Patient’s name, owner last name, site, date, and hospital ID

Coomb's reagent isn't species specific.

false

If a patient tests positive on a Coomb's test, what does it tell us about our patient?

If a patient tests positive on a Coomb's test it tells us that their own antibodies are binding to RBC's. There is also RBC removal by macrophages and intravascular hemolysis which then causes anemia

What red blood cell morphology is typically associated with immune mediated hemolytic anemia?

spherocytes

Which species has naturally occurring antibodies indicating a crossmatch should be performed before every blood transfusion?

Feline

Explain what a novel transfusion is

A novel transfusion is an approach that is new usually when exploring innovative methods for improving blood transfusion effectiveness or reducing harm (patients first transfusion)

Explain the difference between a major crossmatch and a minor crossmatch

In a major crossmatch, it uses the donor's RBCs and the patient's serum, it also then detects major problems. In a minor crossmatch, it uses the patient's RBCs and donor's serum, it also then detects minor problems.

What constitutes a major incompatibility during a crossmatch? (Blank #1 and #2) Can we still transfuse a patient with a major incompatibility? Yes or no. (Blank #3) 

hemolysis and agglutination. No