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Comparative Genomics
Compares the DNA sequences / genomes of different species
Scientists compare differences in DNA sequences and genes
Species with more similar DNA sequences and genes are more closely related and thus share a more recent common ancestor
Species with more differences in their DNA sequences and genes are less closely related and thus have a more distant common ancestor
Differences in the DNA sequence or genes are causes by mutations which accumulate over generations
Protein Sequences
Proteins are made of specific amino acid sequences determined by DNA
Scientists can compare differences in the amino acid sequence of the same protein in different species
Species with more similar amino acid sequence are more closely related and thus share a more recent common ancestor
Species with more differences in their amino acid sequences are less closely related and thus have a more distant common ancestor
Differences in the amino acid sequence are causes by mutations which accumulate over generations
Mitochondrial DNA
Mitochondrial DNA (mtDNA) is small circular DNA found in mitochondria and inherited solely through the maternal line
Scientists can compare differences in the mtDNA sequence between individuals, populations, or closely related species
Species with more similar mtDNA sequences are more closely related and thus share a more recent common ancestor
Species with more differences between their mtDNA sequences are less closely related and thus share a more distant common ancestor
Differences in the mtDNA are causes by mutations which accumulate over generations
Bioinformatics
Combines biology, computer science, mathematics, and statistics to analyse biological data (DNA sequences / genomes, protein sequences, or mtDNA)
Computer programs compare large amounts of this biological data to identify similarities and differences between organisms.
Species with more similar sequences are more closely related and thus share a more recent common ancestor
Species with more differences in their sequences are less closely related and thus share a more distant common ancestor
Process of Fossil Formation
Fossil: preserved remains, impressions or traces of organisms that lived in the past.
Organism dies → soft tissues usually decompose or are eaten by scavengers/microorganisms.
Hard structures such as bones, teeth and shells are more likely to remain.
Remains are rapidly buried by sediment such as mud, sand or silt → reduces decomposition and damage.
More sediment accumulates → becomes compacted into sedimentary rock.
Mineral-rich groundwater can enter remains and deposit minerals → permineralisation preserves their structure.
Fossils can also form as moulds, casts, impressions or traces such as footprints/burrows.
Over time, erosion/geological processes expose the fossil → allowing discovery.
Conditions Required
Fossilisation is rare and requires favourable conditions.
Rapid burial → protects remains from scavengers, physical damage and decomposition.
Low oxygen → reduces activity of decomposers → slows decomposition.
Hard body parts (bones, teeth, shells) → resist decay → more likely to fossilise.
Aquatic/sediment-rich environments are favourable → mud, sand or silt can rapidly cover remains.
Continued sediment deposition + mineral-rich groundwater can assist preservation/permineralisation.
Soft tissue can occasionally be preserved under exceptional conditions such as ice, amber or peat where decomposition is greatly reduced.
Issues with the Fossil Record
The fossil record is incomplete → does not represent every organism that has existed.
Incomplete fossilisation: most organisms decompose because fossilisation requires specific conditions.
Preservation bias: hard-bodied organisms are much more likely to fossilise than soft-bodied organisms.
Destroyed fossils: erosion, Earth movements, heat/pressure and human activity can damage or destroy fossils.
Not discovered: many fossils remain buried and have not yet been found.
Incomplete fossils: often only fragments are preserved → scientists must reconstruct/interpret organisms from limited evidence.
Difficult to date: different dating techniques have particular age/material limitations.
Despite these issues, fossils provide important evidence of evolutionary change over time.
Relative Dating - Definition
Relative dating determines whether a fossil or rock is older or younger than another, providing a chronological order.
Statiagraphy - How it Works, Pros, Cons
How it works
Stratigraphy: studies layers of sedimentary rock called strata.
Fossils are considered approximately the same age as the rock layer in which they occur.
Principle of superposition: in undisturbed sedimentary strata, oldest layers are at the bottom and youngest at the top.
Therefore: lower fossil = generally older; higher fossil = generally younger.
Advantages
Simple way to place fossils into chronological order.
Useful when fossils cannot be directly dated.
Allows relative ages of fossils within strata to be compared.
Disadvantages
Gives relative age only — not a numerical age.
Folding, faulting and erosion can disturb strata.
Missing rock layers can create gaps in the geological record
Index Fossils - How it Works, Pros, Cons
How it works
Index fossils come from species that existed for a relatively short geological time.
Finding the same index fossil in different locations suggests the rock layers are approximately the same age.
Allows scientists to correlate strata between different geographical locations.
Fossilised pollen can act as an index fossil and provide evidence of past vegetation and climate.
Advantages
Allows rock strata from different locations to be compared.
Useful even when corresponding strata occur at different depths.
Some index fossils can also provide information about past environments/climate.
Disadvantages
Requires an appropriate, identifiable index fossil to be present.
Only provides a relative age, not an exact numerical age.
Species must have existed for a limited geological period to be useful.
Absolute Dating
Absolute dating estimates the numerical age of a fossil
Carbon 14 Dating
How it works
Living organisms continually obtain carbon, including radioactive carbon-14 (¹⁴C).
When an organism dies, carbon intake stops.
¹⁴C then progressively decays into nitrogen-14.
Carbon-14 half-life = approximately 5,730 years.
Scientists measure remaining ¹⁴C relative to stable carbon to estimate time since death.
Generally useful for organic material up to ~60,000 years old.
Advantages
Provides an approximate numerical age.
Useful for relatively recent organic remains.
Can directly date suitable biological material.
Disadvantages
Only useful for once-living/organic material.
Not reliable for material much older than ~60,000 years → too little ¹⁴C remains.
Contamination can affect accuracy.
Accelerator Mass Spectrometry (AMS)
How it works
Specialised form of radiocarbon dating.
Directly measures/counts carbon isotopes.
Requires a much smaller sample than conventional radiocarbon dating.
Can date very small quantities of organic material, including material associated with cave paintings.
Advantages
Requires only a very small sample.
Useful when available material is limited or valuable.
Can analyse samples too small for conventional radiocarbon techniques.
Disadvantages
Still uses carbon-14 → similar age/material limitations.
Requires specialised and expensive equipment.
Contamination can affect results.
Potassium - Argon Dating (K - Ar)
How it works
Radioactive potassium-40 (⁴⁰K) decays to argon-40 (⁴⁰Ar).
Potassium-40 half-life ≈ 1.3 billion years.
Long half-life → useful for very old material.
Particularly used to date volcanic rock.
Fossils generally aren't directly dated → scientists date associated volcanic rock and infer the fossil's age.
Advantages
Can date very old material beyond the range of C-14.
Useful for ancient fossils when associated volcanic material exists.
Provides an approximate numerical age.
Disadvantages
Requires suitable volcanic rock/material.
Fossil age is usually determined indirectly from surrounding/associated rock.
Geological alteration or changes in argon can affect accuracy.
Dendrochronology
How it works
Also called tree-ring dating.
Trees generally produce one growth ring each year.
Unknown wood samples are compared with overlapping ring patterns from known-age samples.
Scientists can cross-date matching ring patterns.
Environmental/climatic conditions affect ring width → distinctive rings can act as markers.
Advantages
Can provide very precise dating, sometimes to an individual year.
Does not rely on radioactive decay.
Ring patterns also provide evidence about past climate/environmental conditions.
Disadvantages
Restricted to suitable wood/tree material.
Requires identifiable and overlapping tree-ring patterns.
More limited time range than techniques such as K–Ar dating.