DNA Replication

helicase function

unwinds strands by separating hydrogen bonds bewteen base pairs

topoisomerase function

prevents supercoiling of DNA so that replication fork can continue

DNA polymerase III function

catalyses addition of nucleotides according to the DNA template

RNA primer function

provides a starting point for DNA synthesis

direction of leading strand

5 to 3

ligase function

seals fragments of the lagging strand together

what type of process is DNA replication?

semi conservative process

direction of lagging strand

3 to 5

DNA polymerase I function

removes RNA primers and replaces them with DNA nucleotides

Okazaki fragments

gaps between the lagging strand

enzyme responsible for proofreading errors

DNA polymerase