Lec 14 - Cell junctions & cytoskeleton

Cytoskeleton is the cell _______

skeleton

What are the 3 major families of cytoskeleton

1. actin (dynamic)

2. intermediate (non-dynamic)

3. microtubules (dynamic)

Cytoskeleton is essential for cell _______ and keeps the cell _____

movement, shape

Actin is the ______ microfilament of the 3

smallest

T/F? Actin is dynamic

True

Intermediate filaments are the ______ of the 3

strongest

T/F? Intermediate filaments are not dynamic

True

Microtubules are the ______ filaments of the 3

biggest

T/F? Microtubules are dynamic

True

Phalloidin is a _____ fluorescent that binds to _____ filament

red, actin

What is the function of microvilli?

absorption, increase surface area

Microtubule provide ____ and _____ to eukaryotic cells

structure, shape

T/F? Microtubules shuttle vesicles from one membrane bound organelle to another

True

Two types of cilia?

Motile (9+2 microtubule arrangement)

Non motile (signaling centers)

Congenital ciliary dyskinesia and tubulin mutations is when ______ filament is mutated

microtubule

Intermediate filaments are connected to ______ to stabilized epithelia cells

junctions

What are the 3 domains of the 3d cell

apical

lateral

basal

Apical domain has _____ junctions

tight

• control passage of ions, associated w/ actin filaments

Lateral domain has ____, ___, & ____ junctions

adherens

• cell-cell adhesion, associated w/ actin filaments

desmosomes

• cell-cell adhesion, associated w/ intermediate filaments

gap

• cell-cell communication

Basal domain has _____ junctions

hemidesmosomes

• adherence of epithelia sheets to matrix, associated with intermediate filaments

review cheat sheet

slide 26