CARBOHYDRATES

MONOMERS, POLYMERS, AND CARBOHYDRATES.

EXPLAIN WHAT THE DIFFERENCE IS BETWEEN ALPHA AND BETA STRUCTURES?

DEFINE THIS MONOSACCHARIDE.

DIFFERENCE: Beta = Carbon-1’s hydroxyl points upwards.

GLUCOSE: Photosynthesis’s product and respiration’s substrate.

CARBOHYDRATES

CARBS ARE KEY BIO MOLECULES THAT STORE GLUCOSE AND CAN PROVIDE STRUCTURAL SUPPORT TO PLANT CELLS.

MONOSACCHARIDES QUALITIES + FORMULA

WHAT IS THE BOND IN DISACCKS?

• THEY ARE SOLUBLE AND SWEET.

• C6H1206

• ALPHA 1-4 GLYCOCICYDIC BOND.

STARCH STRUCTURE:

1. • AMYLOSE FORMS A HELICAL CHAIN (MAKING IT COMPACT) TO STORE LARGE NUMBERS OF GLUCOSE MOLECULES IN A SMALL SPACE.

   • AMYLOPECTIN IS BRANCHED, PROVIDING A LARGE SURFACE AREA FOR ENZYMES TO ATTATCH. THIS MEANS THAT STARCH IS READYILY HYDROLISED BACK INTO GLUCOSE WHEN PLANT CELLS ARE RUNNING LOW ON GLUCOSE FOR RESP.

   • ALPHA 1-6 BOND CAUSES BRANCHING

GLYCOGEN

1. JOB?

2. POLYMER OF?

3. WHERE IS IT FOUND?

1. ENERGY STORAGE MOLECULE IN ANIMALS AND FUNGI.

2. POLYMER OF GLUCOSE: SO ITS EASILY HYDROLISED AND PROVIDES RESPIRITORY SUBSTRATE FOR ENERGY RELEASE.

3. LIVER AND MUSCLE CELLS.

Highly branched.

WHY ARE STARCH AND GLYCOGEN BOTH WELL SUITED AS GLUCOSE STORAGE MOLECULES?

1. THEY ARE COMPACT AND SO STORE LOTS OF GLUCOSE IN A SMALL SPACE.

2. THEY ARE LARGE AND INSOLUBLE AND HAVE NO OSMOTIC EFFECTS.

3. THEY ARE RELETIVELY INERT AND SO DO NOT BECOME INVOLVED IN CHEMICAL REACTIONS IN THE CELL.

4. MANY ENDS ::: EASY TO HYDROLYSE.

SIMILARITIES BETWEEN STRUCTURE OF STARCH AND CELLULOSE:

1. Both polysaccharides; OR Both are glucose polymers OR Both are made of glucose monomers

2. Both contain glycosidic bonds (between monomers).

3. Both contain carbon, hydrogen and oxygen.

WHY IS CELLULOSE SUITED TO ITS JOB?

It comprises beta glucose chains, forming straight, unbranched chains (linear polymer).

STARCH TEST

1. SOLVENT?

2. OG COLOUR?

3. POSITIVE COLOUR?

1. IODINE

2. BROWN/ORANGE

3. BLUE-BLACK

WHAT IS A REDUCING SUGAR?

ABILITY TO DONATE ELECTRONS.

ALL MONOSACCHS AND SOME DISACCHS ARE REDUCING SUGARS.

NON-REDUCING SUGAR TEST

1. STEPS

N-RS EG: SUCROSE

1. Heat with acid and neutralise.

2. Heat with Benedict's.

3. Red precipitate/colour.

ISSUES WITH THE BENEDICTS TEST AND HOW TO FIX THEM

1. NON-SPECIFIC: A BIOSENSER E.G. GLUCOSE BIOSENSER CAN BE USED.

2. QUALIATIVE: COLOUR CHANGE DETERMINES RESULT, SO WE CANNOT OBTAIN A VALUE FOR [REDUCING SUGAR]. COLOUR CHANGE DEPENDS AMOUNT OF REDUCING SUGAR, ::: TEST IS SEMI-QUALIATIVE, ALLOWING EST OF HOW MUCH REDUCING SUGAR IS PRESENT:

   • BLUE - NONE

   • R - HIGH

3. SUBJECTIVE. COLOUROMITER CAN QUANTIFY RESULTS.

HOW DOES A COLOUROMITER IMPROVE THE REPEATABILITY OF RESULTS?

1. RESULTS ARE SUBJECTIVE.

2. THIS STANDARDISES THE METHOD.

APART FROM A COLOUROMITER, HOW ELSE CAN YOU MEASURE THE QUANITITY OF REDUCING SUGAR IN A SOLUTION?

1. FILTER AND DRY THE PRECIPITATE.

2. FIND MASS/WEIGHT.

A quantitative Benedict’s test produces a colour whose intensity depends on the concentration of reducing sugar in a solution. A colorimeter can be used to measure the intensity of this colour. The scientist used quantitative Benedict’s tests to produce a calibration curve of colorimeter reading against concentration of maltose. Describe how the scientist would have produced the calibration curve and used it to obtain the results in the graph. Do not include details of how to perform a Benedict’s test in your answer.