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A proces called was refined by which revolutionized the biotech industry by allowing rapid accurate copying of DNA

PCR, Mullins

Type of PCR that allows the operator to measure amplicons real time is called

qPCR ( real-time)

polymerase is an enzyme that makes DNA copies from the leading strand ( to )

DNA, 5-3

A —— is a complimentray sequence of 16-20bp that is added to the forward and reverse strands during annealing

RNA primer

Type of pcr that makes cDNA from mRNA is called

RT-PCR

A mix of and are added to the master mix during the chain termination method. A DNA squencing machine gives the final sequence based on both and mass of each amplicon

dNTPs and ddNTPs, Florescence

what is an enzyme used during extension in traditional PCR

taq polymerase

the ability to quickly cycle temperatures in a thermocucler is accomplisjed with the useof a block

peltier

a marker is added to ddNTPs to aid in seuencing

flourescent

the product of PCR is called an

amplicon

the. is a database of STR patterns ised by the FBI to identify repaet offenders of crimes

CODIS

are added to the growing chain during extension

dNTPs (nucleotides)

in forensic applications, the type of PCR that would be best to amplifiy multiple STR targets in a sample is

multiplex PCR

a. is a device that a PCR reaction occurs in

thermocycler

the diagnosis of COVID is definitively diagnosed with a combination of and. PCR methods

RT-PCR and qPCR

type of eukaryitic template DNA most apporpriate of use with prokaryotes

cDNA

a series of primers that differ by a couple of nucleotides is added to when the target is not well known

degenerative PCR

is a solution used to digest plasma and nuclear membranes to releases eukaryotic DNA

Instagene Matrix

A cocktail of enzymes and solutios added to a sample, for PCR, is commonly called

mastermix

what is performed when the target DNA is suboptimal andd may need a second sequence of degenerative and conventional PCR

Nested PCR

the iconic compund is added to master mix to stailize DNA and improve the PCR yield

MgCl2

A DNA. consist of plate annealed with multiple probes that flouresce when complimentary nucleotides are present

microarray

what are variable length segment of DNA used in DNA fingerprinting with PCR

STRs

In an engineered plasmind, and. primer sites flank the MCS area to be used in verification of insertiom of rDNA

reverse and forward

inorder to digest the root bulb to expose the plasma membrane, the enzyme. is required

protease

what is the name given to the process of identifying bacteria by amplifying the DNA used to assemble the small unit of the

DNA Barcoding, ribosome

dNTPs are deoxy at the _C while ddNTPs are also deoxy at the _C

2’, 3’

PCR reactions like those done in this class are referred to as _ PCR

conventional

Amplifying DNA found in the is used to trace meternal lineage through services such as 23andMe

mitochondria

DNA that the primer searches for is called the

target

Name and temperature of stage A

Denaturation; T= 94C

Name and temperature of stage B

Annealing; T= 55C

Name and temperature of stage C

Extension; T=72C