Bio Lab Post lab #1

What is the slope formula?

y = mx + b

What did the experiment study?

data on total protein concentration in stigmas (structure where pollen grain attach) of different species of lilies or different varieties of lilies, and analyzing the data statstically

What was the experiment measuring?

protein concentration in diff stigmas

What was the indicator used?

Bradford reagent, contains the compound Coomassie blue, turns blue in the prescence of proteins

What wavelength was the spectrophotometer set to?

595 nm

How to determine if a hypothesis is supported?

If the data collected during the experiment has enough statistical significance to prove the null hypothesis incorrect

How do you use a spectrophotometer?

Turn on device → set to the correct wavelength → calibrate machine with a blank/control sample → measure test solutions → in between every sample place the control sample back into machine to recalibrate it

How do you use a pipette?

set micropipette to proper volume on the dial → attach a disposable tip → press plunger to the first stop → draw the liquid slowly → press to the second stop to dispense

P-value

stands for “probability value”, in chemistry our P-value is 0.05.

• If the calculated P-value is LESS than 0.05, the results are statistically significantly different (meaning data is good).

• If the calculated P-value is GREATER than 0.05, the results are NOT statistically significantly different (data is not good)

What was the conclusion and P-value we got during the lab?

A P-value of .004, meaning it is < 0.05, indicating that stigma protein concentration in different varieties of lilies is significantly different