Microbiology Quiz 4 Dr. Collins Cedarville University
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40 Terms
What is a positive result of a catalase test?
bubbles
What is the negative result of catalase activity?
no bubbles
What is the catalase equation?
2H202--->2H20 + O2
What kind of enzymes produce catalase activity?
obligate aerobes and facultative anaerobes
What is a positive reaction of urease?
pink color
What indicates a negative reaction of urease?
orange color
What is a positive reaction for coagulase activity?
clumping in the test tube
What is a negative reaction for coagulase?
no clumping in test tube
How is coagulase advantageous to a microorganism?
causes the clotting of blood plasma
clot-forming threads formed at the site of an injury during coagulation where platelets clump together with various other substances
fibrin clot
What indicates a positive result for oxidase activity?
sample turns purple
What is the result of a negative oxidase activity test?
no color
What is the function of the substrate of oxidase activity?
acts as an electron donor transferring electrons to cytochrome c oxidase and becomes oxidized
What is a positive reaction for an EMB test for lactose fermentation?
black or dark center colonies or green metallic colonies
What is a negative reaction for the EMB test for lactose fermentation?
clear to white colonies
What indicates a positive result for indole?
pink or red ring at top of test tube
What indicates a positive test for Methyl-red test?
media is red
What indicates a positive test for citrate?
media is blue
What indicates a negative methyl-red test?
Media is orange
What indicates no change in a TSI slant?
no change (NC)
What is indicated for alkaline in a TSI slant?
K, red color
What is used to indicate acid in a TSI slant?
A, yellow color
What indicates CO2 production of a TSI slant?
gas production, crackles or bubbles in the deep
What indicates H2S in a TSI slant?
hydrogen sulfate, black precipitate
K/A indicates what? (red/yellow)
glucose fermenter
A/A indicates what? (yellow)
Glucose, Lactose/sucrose fermenter
What does K/K indicate? (Red/red)
Non-fermenter, peptones utilized
What does K/NC indicate? (Red/no change)
aerobic non-fermenter-peptones are used aerobically
What indicates a positive caries activity?
media turns yellow
What indicates negative caries activity?
green media
What is the media composed of for dental caries?
glucose and bromocresol green
What is the equation to determine the pff/ml of a tube of bacteriophage?
pfu/ml= pfu x dilution factor/volume (ml)
What type of phage was used in the bacteriophage lab?
lytic
viruses that use bacterial cells to replicate
lytic
Process in which new viral particles are released from the bacterial cell
lysis
pfu stands for what?
plaque foriming units
clear zones on an agar are what?
plaques
the process used to make yogurt
lactic acid fermentation
microorganism contained in yogurt
probiotics
process used to the make food products
fermentation