Week 13 - Electrophoresis

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Last updated 9:15 AM on 5/11/26
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9 Terms

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2 Dyes

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Nucleic Acids Charge

Negative

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Proteins Charge when pH < pI

Positively charged

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Proteins Charge when pH > pI

Negatively charged

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Native PAGE

Separate by size/shape/charge

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SDS-PAGE

Detergent denature proteins

Separate by weight

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PAGE

Polyacrylamide Gel Electrophoresis

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Isoelectric Point pI

pH at which protein has no net charge

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Isoelectric Focusing IEF - Process

Create pH gradient

Proteins migrate until reach pI

Separate by charge