bmic topic 3,3 microbiological media: functional types of media

part 3

what are the different types of functional media?

general minimal selective differential

what is general media?

support the growth of a wide variety of microorganisms except fastidious species

what is fastidious species?

nutritionally demanding wide variety of growth factors needed

what are some examples of general media?

nutrient broth agar tryptone soya broth agar

how is minimal media defined?

chemically defined

what does minimal media contain?

contain minimum nutrients to support the growth of a specific species

what is minimal media useful for?

useful for screening of auxotrophic mutants and to investigate the minimal nutritional requirements of the organism and defined them

what type of products does minimal media contain?

usually only contains a C source inorganic salts water no amino acids are provided

what does selective media contain?

contain a specific inhibitory substances against specific microorganisms

what does the inhibitory substances in selective media do?

suppresses the growth of undesired microorganisms and favor the growthof particular microorganisms

what does selective media allow?

allow rapid isolation as well as preliminary identification of desired microorganisms

what does differential media do?

give visual differentiation by appearance to distinguish colonies of 1 microorganism from another

what does differential media involve?

usually involve nutrient utilization

what happens if acid/alkali production is involved in differential media?

if acid/alkali production is involved pH indicator is always included

what is differential and selective media?

possess both differential and selective properties

what is differential and selective media used for?

often used for presumptive identification of microorganisms from food or clinical samples

what are some examples of differential and selective media?

MacConkey agar mannitol salt agar

media for viruses: what are viruses?

viruses are intracellular obligate parasites

what must be provide in the media for viruses?

bacterial cell cultures
living animal or plants
tissue or cell cultures