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What is the primary use of Brightfield microscopy?
To view stained bacteria.
What are the drawbacks of Brightfield microscopy?
It can only view dead specimens and cannot view viruses.
What is the advantage of using Darkfield microscopy?
It allows viewing of smaller live bacteria.
What can DIC microscopy reveal about live specimens?
It provides '3D' contrast and can view eukaryotic organelles.
What is the function of Fluorescence microscopy?
It uses fluorophores to view proteins and molecules.
What is TIRF microscopy used for?
To view proteins and molecules at the membrane of cells.
What is a significant limitation of both Fluorescence and TIRF microscopy?
Neither can view viruses.
What is the purpose of Transmission Electron Microscopy (TEM)?
To view internal structures at the nanometer scale.
What is a key drawback of Transmission Electron Microscopy (TEM)?
It can only view dead specimens and cannot view viruses.
What does Scanning Electron Microscopy (SEM) allow researchers to observe?
It allows viewing of surfaces using electron beams.
What is a limitation of Scanning Electron Microscopy (SEM)?
It can only view dead specimens.