Microscopy Techniques and Their Applications

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11 Terms

1
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What is the primary use of Brightfield microscopy?

To view stained bacteria.

2
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What are the drawbacks of Brightfield microscopy?

It can only view dead specimens and cannot view viruses.

3
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What is the advantage of using Darkfield microscopy?

It allows viewing of smaller live bacteria.

4
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What can DIC microscopy reveal about live specimens?

It provides '3D' contrast and can view eukaryotic organelles.

5
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What is the function of Fluorescence microscopy?

It uses fluorophores to view proteins and molecules.

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What is TIRF microscopy used for?

To view proteins and molecules at the membrane of cells.

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What is a significant limitation of both Fluorescence and TIRF microscopy?

Neither can view viruses.

8
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What is the purpose of Transmission Electron Microscopy (TEM)?

To view internal structures at the nanometer scale.

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What is a key drawback of Transmission Electron Microscopy (TEM)?

It can only view dead specimens and cannot view viruses.

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What does Scanning Electron Microscopy (SEM) allow researchers to observe?

It allows viewing of surfaces using electron beams.

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What is a limitation of Scanning Electron Microscopy (SEM)?

It can only view dead specimens.