Lab Homework #2- Approach to the Blood Film, Normal Red Blood Cell Morphology (Domestic Species)

Identify and state the function of the parts of the microscope that the arrows are pointing to

Turret/Revolving Nosepiece (Red Arrow)

- The part of the microscope that holds the objectives. It can be rotated to change to a different objective.

Condenser (Blue arrow)

- Use of a condenser allows further control of illumination of the specimen, as well as, enhancement of the resolution.

Field Diaphragm (Purple arrow)

- Regulates the amount of light entering the condenser

Identify and state the function of the parts of the microscope that the arrows are pointing to

Focus adjustment ring (pink arrow)

-It allows focus adjustments to be made to the eyepiece to compensate for differences in vision in the two eyes of
the user so both eyepieces may be comfortably used

Coarse focus knob (green arrow)

-Used with the low power objective to initially bring the objects into focus. It moves the objective towards or away from the specimen on the stage.

Stage movement control knob (Blue arrow)

- Moves stage forward and backward and slide holder left to right.

Identify, label and provide a written description of how the area would appear visually through the microscope of the three main areas on this peripheral blood film.

1.) Feathered Edge (end of the curved line)
- When looked at through the microscope you will see streaks, stripe appearance. There are large white spaces

2.) Monolayer (slightly past the curved line)
- Red blood cells are close to one another but not overlapping

3.) Body of the smear (close to the "case number end")
- Less white space than the monolayer, there is stacking that can be seen, and lots of crowding

Llamas have red blood cells that are _______ in shape

oval/elliptical

Which of the domestic species has the smallest red blood cells?

Caprine

Which tube doesn't contain clotting factors and fibrinogen?

Sodium citrate, serum, EDTA, or Heparin

Serum

Erythropoietin

protein that stimulates production of red blood cells

Thrombopoietin

Protein that stimulates production of platelets

Granulocyte Colony Stimulating Factor

Protein that stimulates production of white blood cells

What is the difference between hematopoiesis and erythropoiesis?

Hematopoiesis is the formation and development of blood cells in the bone marrow but erythropoiesis is the formation of red blood cells specifically.