micro ch 9/10--biotechnology/identification and classification of prokaryotes

some uses of genetic engineering

cancer treatment

DNA typing

food production

insulin/other medications

in vitro

study of living components in glass

in vivo

studies done in living organisms

vector

a carrier, could be plasmic/viral or an animal

reverse transcriptase

an enzyme that makes DNA from mRNA

cDNA/complimentary DNA

new DNA made from old mRNA

transgenic organism

plants or animals that contain and foreign gene

gel electrophoresis

when you take an unknown substance and put it into a gel

electrical current is applied and causes the substance to move through the gel

forms bands

PCR/polymerase chain reaction

process of rapidly copying DNA by heating it until it separates

thermophile

enzyme that copies stands over and over

probe hybridization

when you take a single stranded piece of DNA and match it to a piece

colony blotting

where bacteria are grow on a petri plate on a nylon membrane

picks up the colonies the cells are broken with a chemical and a probe of interest is added to see which colonies had that particular DNA

FISH

when you take cells that are put on a slide and add fluorescents for the picture

DNA microarray

when you put single stranded genes attached to a slide/computer chip

taxonomy

science of classification

morphology

the study of shape

staining

does the bacteria stain G-, G+ or acid fast

biochemistry

does the bacteria ferment glucose or other carbs?

serology

antibody reactions

does this bacteria react with any known antibodies

phage typing

has this bacteria been infected by a known phage

amino acid sequencing

what bacteria is this bacteria mad of?

DNA analysis

what are this bacteria’s DNA bases?