Lab safety and equipment

Example of a zoonotic disease

All the above

Zoonotic

Infectious disease that can be transmitted between animals and humans

What goes in sharps ?

Needles, glass

What goes in Biohazard ? 

Blood, used tissues

What goes in Sink brain ?

Water

What goes in Trashcan

Wrappers, trash, paper

Proper way to clean a microscope

Clean stage and knobs with an alcohol soaked tissue. Spray lens cleaner on a tissue and clean ocular and objective lens.

Proper way to clean a centrifuge

Clean external surfaces with a soft cloth with neutral detergents

What is the purpose of a centrifuge ?

Used to separate substances of different densities

Can you use the same centrifuge for every lab sample?

Yes

SDS

Safety Data Sheets

What is the SDS binder ?

Collection of all materials presents in the clinic, know where is it at.

How would you appropriately appreciate the smell of an unknown substance ? 

Wafting technique

Clerical

• Most common error

• incorrect sampling

• incorrect labeling

• incorrect transport

Random

• Glassware imperfections

• equipment failure

• temperature variables

• timing errors

Systemic

• Inaccurate standard controls

• reagent instability/expired

• handling errors

• negligence to protocol

VAs are NOT to

Do final interpretation of results

OSHA

Occupational Safety and Health Administration

When was OSHA est ?

1971

What does OSHA do ?

Sets and enforces workplace safety standards

PPE

Personal Protective Equipment

4 types of pathogens

1. Protozoan

2. Fungi

3. Bacteria

4. Virus

Ringworm is a _____

Fungi

Rabies is a _____

Virus

Routes of transmission from zoonotic diseases

Direct and indirect

Direct transmission

• Bites

• Fluid, urine

• infectious tissue

Indirect transmission

• Airborne

• Vector

• Food and water

• Fecal-oral

What three main equipments do we use ?

1. Refractometer

2. Centrifuge

3. Microscope

Refractometer

Used to test urine concentration and serum protein

Which one of the centrifuges would you use with hematocrit tubes ?

A

Which two of the centrifuges would you use with serum separator tubes ? 

B and C

Clot activator

facilitates blood clotting

• Yields Serum

Anticoagulant

Prevents blood from clotting

• Yields Plasma

Serum

the liquid portion of blood that remains after coagulation, containing no cells or clotting factors.

Plasma

the liquid component of blood that remains after clotting occurs, containing water, electrolytes, and proteins.